| 农产品/食品中农药残留快速检测方法研究进展 |
| |
| 引用本文： | 蒋雪松,王维琴,许林云,卢利群,周宏平,陈卉卉.农产品/食品中农药残留快速检测方法研究进展[J].农业工程学报,2016,32(20):267-274. |
| |
| 作者姓名： | 蒋雪松 王维琴 许林云 卢利群 周宏平 陈卉卉 |
| |
| 作者单位： | 1. 南京林业大学机械电子工程学院，南京 210037; 宁波海通食品科技有限公司，宁波 315300;2. 宁波海通食品科技有限公司,宁波,315300;3. 南京林业大学机械电子工程学院,南京,210037 |
| |
| 基金项目： | 江苏高校优势学科建设工程资助项目（PAPD）；南京林业大学青年科技创新基金（CX2015010）；江苏省高校优秀中青年教师和校长境外研修资助项目》（苏教办师﹝2015﹞7号） |
| |
| 摘 要： | 农药残留的识别和量化通常依赖于气相色谱法、高效液相色谱法、气/液相色谱-质谱联用法以及毛细管电泳法,这些方法需涉及大而贵重的仪器、费时的样品处理以及专门的技术培训。因此,建立在线、高灵敏度、高选择性、简单高效、低成本的农药残留快速检测方法和技术非常重要。该文综述了用于农产品/食品的农药残留分析快速检测方法,主要包括酶抑制法、免疫分析法、光谱法(包括可见/近红外、红外、拉曼和激光诱导击穿光谱等)以及各种生物传感器等,分别介绍了这些方法最新的研究进展,同时分析并总结了这些快速检测方法和技术的基本原理和特点。目前的研究在灵敏度、重复性、准确性方面存在着一些不足,商品化的农药残留检测仪器也比较单一。由于纳米生物技术、分子印迹技术和微流控技术等技术有着巨大的应用潜力,因此特别介绍了这些技术在农药残留分析中的应用。农药残留快速分析技术未来将会朝着检测仪器的小型化和集成化、多通道检测、无线通讯方向发展,提高快速检测方法和仪器的稳定性和可靠性是必然趋势。
|
| 关 键 词： | 农产品 农药 光谱分析 快速检测 酶抑制法 免疫分析 生物传感器 |
| 收稿时间： | 2016/6/19 0:00:00 |
| 修稿时间： | 2016/9/26 0:00:00 |
Review on rapid detection of pesticide residues in agricultural and food products |
| |
| Jiang Xuesong,Wang Weiqin,Xu Linyun,Lu Liqun,Zhou Hongping and Chen Huihui.Review on rapid detection of pesticide residues in agricultural and food products[J].Transactions of the Chinese Society of Agricultural Engineering,2016,32(20):267-274. |
| |
| Authors: | Jiang Xuesong Wang Weiqin Xu Linyun Lu Liqun Zhou Hongping and Chen Huihui |
| |
| Institution: | 1. College of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Ningbo Haitong Food Science & Technology Company Limited, Ningbo 315300, China,2. Ningbo Haitong Food Science & Technology Company Limited, Ningbo 315300, China,1. College of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, China,2. Ningbo Haitong Food Science & Technology Company Limited, Ningbo 315300, China,1. College of Mechanical and Electronic Engineering, Nanjing Forestry University, Nanjing 210037, China and 2. Ningbo Haitong Food Science & Technology Company Limited, Ningbo 315300, China |
| |
| Abstract: | Abstract: Pesticides were widely applied to eliminate or control a variety of agricultural pests and diseases. However, they exhibited acute or chronic toxicity on human health through their residues in agricultural product and food. Thus, monitoring pesticide residues was extremely crucial to ensure that pesticides in agricultural products were in permitted levels. The identification and quantification of pesticides were generally based on mass spectrometry combined with gas and/or liquid chromatography, or capillary electrophoresis. However, these methods involved large and expensive instruments, time-consuming sample preparation and trained personnel. Therefore, it was important to set up onsite assays and techniques, especially highly sensitive and selective, simple, efficient, and cost effective ones for rapid detection of pesticide residues. This review summarized the application of fast analytical methodologies such as enzyme inhibition assays (EIA), immunoassays, optical spectroscopy assays and various biosensors in the analysis of pesticide residues in agro product and food in the past few years. EIA was widely used for rapid colorimetric or spectrophotometric screening of pesticides in China, which was based on the inhibition of cholinesterase (ChE) activity by organophosphate and carbamate. Immunoassays, such as enzyme-linked immunosorbent assay (ELISA), were quantitative or qualitative methods of analysis for a substance, which took antibodies as the analytical reagents. Immunoassays depended on the use of an analytical reagent that was associated with a detectable marker, such as enzyme, fluorescence, chemoluminescence radioactive element, and so on. Colloidal gold-based immunoassay was proven to be fast, sensitive and cost-effective for pesticide detection. Biosensors were analytical devices, used for the detection of analytes, which combined a biological component with a physicochemical detector. The development of biosensors for pesticides was also an active research area, which offered great advantages over conventional analytical techniques. Aptamers were shown as good candidates to replace the conventional antibodies in more robust and stable biosensors for pesticide detection. Optical spectroscopy assays, including visible/near-infrared, Roman, and Tera Hertz time-domain spectroscopy, had pollution-free and lossless nature, which were regarded as potential methods for qualitative and quantitative analyses of pesticides. The basic principles of these fast screening strategies were discussed, and both advantages and drawbacks of these techniques were then summarized. Special emphasis was placed on the state-of-art techniques including nanobiotechnology, molecular imprinting technique (MIT) and microfluidics, because of their great potential in pesticide residue analyses. Nanomaterials including carbon nanotubes (CNTs), quantum dots and nanoparticles were developed to improve the performance of pesticide detection. MIT, as a burgeoning powerful technology, was gaining increasing attention for its prospect of creating synthetic polymers with highly specific recognition capabilities in complicated samples. Furthermore, the future perspectives and the trends for pesticide residue analysis were also presented. Most studies were focused on the detection of insecticides, which were used in very large quantities. In future, it is necessary to pay more attention to the detection of other families of pesticides with lower mammalian toxicity, such as herbicides and plant growth regulators. The miniaturization and integration, multi-channel detection, wireless communication, higher stability and repeatability of the detection instruments are the development direction of pesticide residue detection in the future. |
| |
| Keywords: | agricultural products pesticides spectrum analysis fast detection enzyme inhibition assay immunoassay biosensors |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《农业工程学报》浏览原始摘要信息 |
| 点击此处可从《农业工程学报》下载免费的PDF全文 |
|
