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RAPD Technique Used to Determine the Purity of Hybrid Hot Pepper Seeds |
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| Authors: | Zhou Qunchu He Qing Ma Yanqing Zhang Zouqing Li Xuefeng Ai Xin |
| Abstract: | Two hybrid hot pepper varieties Xiangyan 5 and Xiangyan 10, and their parents were analyzed the polymerase chain reaction with MJ /PT 200 Peltrier Themal Cycler and DS 800 White-ultravilot Transilluminator to set up a RAPD system adaptable to the purity determination of the hybrid seeds. Among the 39 random primers, 2 and 4 primers were found to be used effectively in Xiangyan 5 and Xiangyan 10 respectively. |
| Keywords: | Hot pepper (Capsicum annum) Randomly amplified polymorphic DNA(RAPD) Purity determination |
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