| 转基因大豆种子、豆粕定性PCR检测方法的研究与应用 |
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| 引用本文： | 田雷,王辉,律宝春,贾希海,郑渝.转基因大豆种子、豆粕定性PCR检测方法的研究与应用[J].种子,2004,23(5):28-31. |
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| 作者姓名： | 田雷 王辉 律宝春 贾希海 郑渝 |
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| 作者单位： | 北京市种子管理站,北京,100088 |
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| 摘 要： | 利用定性PCR检测方法,以大豆凝集素基因(Lectin)为内标基因,检测了转基因大豆种子、豆粕中的四个外源基因:35S-CTP基因、Cp4-epsps基因、nos终止子基因、CaMV35S启动子基因,并通过酶切(XmnI)验证试验验证了定性PCR检测结果的准确性,建立了定性PCR检测转基因大豆种子、豆粕的方法,利用市场上抽检到的大豆种子、豆粕样品,进行了实际检测工作.
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| 关 键 词： | 转基因大豆种子、豆粕 定性PCR检测 酶切验证 |
Methodological Research and Application on Qualitative PCR Detection of Genetically Modified Soybean Seeds and Bean Meal |
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| Tian Lei,Wang Hui,Lu Baochun,Jia Xihai,Zheng Yu.Methodological Research and Application on Qualitative PCR Detection of Genetically Modified Soybean Seeds and Bean Meal[J].Seed,2004,23(5):28-31. |
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| Authors: | Tian Lei Wang Hui Lu Baochun Jia Xihai Zheng Yu |
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| Abstract: | The purpose of this study is to establish detection method on genetically modified soybean seeds and bean meal using qualitative PCR technology. This method detects the four major foreign genes in commercial genetically modified soybean seeds and bean meal 35S-CTP, Cp4-epsps, CaMV35S promoter and NOS terminator with soybean lectin as the interior gene. Primers were designed to amplify parts of the 35S-CTP, the Cp4-epsps, the CaMV35S promoter, the NOS terminator and soybean lectin. Lectin primer co-amplified with the above four pairs of primers respectively in the same PCR tube. XmnI validation experimentation confirmed the validity of the PCR result. Samples selected randomly from the market were detected using the above method and the result showed that established qualitative PCR detection methods are sensitive. |
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| Keywords: | Genetically modified soybean seeds and bean meal Qualitative PCR detection XmnI validation |
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