| 机械分割的小鼠桑椹胚和囊胚的发育能力 |
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| 引用本文： | 王周吉,Alan Trounson.机械分割的小鼠桑椹胚和囊胚的发育能力[J].东北农业大学学报,1992(3). |
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| 作者姓名： | 王周吉 Alan Trounson |
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| 作者单位： | 东北农学院，澳大利亚莫那什大学 哈尔滨 150030 |
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| 摘 要： | 利用机械方法二等份分割小鼠的桑椹胚、早期囊胚和晚期囊胚,并在体外培养分割的半胚到晚期囊胚。将这些囊胚移植到假孕受体鼠子宫内以评价他们附植后发育的能力。在体外培养中发现,囊胚期分割的胚胎比桑椹胚期分割的胚胎有较高的成活率。通过附植前胚胎细胞数量分析得知,囊胚期半胚的细胞数量大约是对照组的一半,但内细胞团细胞与整个胚胎细胞的数量比不变。虽然从半胚发育到囊胚期的胚胎附植率仅略低于对照组,但半胚形成胎儿的能力却显著低于全胚。组织学分析表明,附植后的这种低成活率现象是由于缺乏卵柱发育造成的,而此卵柱则与半胚中的内细胞团的细胞数量减少有关。移植到第3天假孕受体鼠中的半胚,附植后的发育情况明显地比移植到第4天受体中的好。
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| 关 键 词： | 小鼠 桑椹胚 囊胚 机械分割 发育能力 |
DEVELOPMENTAL CAPACITY OF MECHANICALLY BISECTED MOUSE MORULAE AND BLASTOCYSTS |
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| Wang Zhouji.DEVELOPMENTAL CAPACITY OF MECHANICALLY BISECTED MOUSE MORULAE AND BLASTOCYSTS[J].Journal of Northeast Agricultural University,1992(3). |
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| Authors: | Wang Zhouji |
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| Abstract: | Mouse embryos were mechanically bisected at the morula, early blastocyst or expanded blastocyst stages of development and cultured in vitroto the expanded blastocyst stage. Their capacity for postimplantation development was assessed after transfer to pseudopregnant foster mice. Embryos bisected at the blastocyst stage had a higher survival rate in vitrothan those bisected at the morula stage. Half-embryos had approximately half the number of cells control embryos at the blastocyst stage, but the propor-tion of cells in the inner cell mass (ICM)to those in the whole embryo was unaltered. The implantation rate of blastocysts derived from bisected embryos was only slightly lower than that of control embryos, but bisected embryos had a significantly reduced capacity to form fetuses. Histological analyses showed that failure to form a fetus is due to the absence of egg cylinder development, which correlates with the reduced number of cells in the ICM of bisected embryos. Postimplantation viability of half-embryos was significantly higher when blastocysts were transferred to Day-3 rather than Day-4 pseudopre gnant recipients. |
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| Keywords: | mouse morulae blastocyst machanicall bisected developmental capacity |
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