| 小金海棠MxYSL1基因的克隆与表达分析 |
| |
| 引用本文： | 刘丽丽,许雪峰,孔瑾,王忆,李天忠,韩振海.小金海棠MxYSL1基因的克隆与表达分析[J].农业生物技术学报,2009,17(2):288-293. |
| |
| 作者姓名： | 刘丽丽 许雪峰 孔瑾 王忆 李天忠 韩振海 |
| |
| 作者单位： | 中国农业大学园艺植物研究所/北京市果树逆境生理与分子生物学重点实验室,北京,100193 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，高等学校博士学科点专项科研基金 |
| |
| 摘 要： | 小金海棠属于铁高效基因型。为了研究小金海棠的抗缺铁分子机理，根据苹果的EST库得到一个791 bp的YSL(yellow stripe 1-like protein)基因片段序列，设计特异引物，在苹果铁高效基因型小金海棠（Malus xiaojinenesis）中克隆到此基因片段。3'-RACE得到YSL基因的3'端序列，拼接后得到1 114 bp的3'端基因序列。预测该基因片段编码一个含7个跨膜区的蛋白多肽，与拟南芥AtYSL1同源性达74%，命名为MxYSL1。RT-PCR及Northern blot分析表明，该基因在小金海棠各个检测部位都有表达。在根、茎与成熟叶中，该基因在低铁(EDTA-NaFe,4 μmol/L)时减弱表达，在过量铁(EDTA-NaFe,320 μmol/L)供应时增强表达。MxYSL1基因在新叶中的表达趋势与以上器官中的表达趋势正好相反。
|
| 关 键 词： | 小金海棠 MxYSL1 铁 RT-PCR Northern blot分析 |
| 收稿时间： | 2008-5-4 |
| 修稿时间： | 2008-5-28 |
Cloning of MxYSL1 Gene in Malus xiaojinensis and Its Expression Analysis |
| |
| LIU Li-li,XU Xue-feng,KONG Jin,WANG Yi,LI Tian-zhong,HAN Zhen-hai.Cloning of MxYSL1 Gene in Malus xiaojinensis and Its Expression Analysis[J].Journal of Agricultural Biotechnology,2009,17(2):288-293. |
| |
| Authors: | LIU Li-li XU Xue-feng KONG Jin WANG Yi LI Tian-zhong HAN Zhen-hai |
| |
| Abstract: | Malus xiaojinensis is an iron-efficient apple genotype. Through the analysis of apple’s EST, a 791 bp YSL (yellow stripe 1-like protein) gene fragments were obtained, and their specific primers were designed to obtainYSL gene fragment in Malus xiaojinensis . The complete 3' sequence of YSL gene was obtained by RACE method, which were composed of 1 114 nucleotides. The predicted amino-acid sequence included 7 putative membrane-spanning domains and its homology with AtYSL1 was up to 74%, and was named as MxYSL1. The results of semi-quantitative RT-PCR and Northern blotting analysis showed that MxYSL1 was expressed in all organs of M. xiaojinensis. The expressions were down-regulated in roots, stems and mature leaves with Fe deficiency treatments(EDTA-NaFe,4 μmol/L), but up-regulated with Fe excess treatments(EDTA-NaFe,320 μmol/L). The young leaves had opposite expression model. |
| |
| Keywords: | MxYSL1 RT-PCR |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《农业生物技术学报》浏览原始摘要信息 |
|
