| 精子整合外源DNA的分子机制与转基因动物的制备 |
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| 引用本文： | 孙玉江,王丽,李兰,潘庆杰.精子整合外源DNA的分子机制与转基因动物的制备[J].中国农业科技导报,2005,7(4):38-40. |
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| 作者姓名： | 孙玉江 王丽 李兰 潘庆杰 |
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| 作者单位： | [1]东营市农业科学研究所,山东东营257091 [2]东营市环保科研所,山东东营257091 [3]莱阳农学院动物科技学院,山东莱阳265200 |
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| 基金项目： | 国家高技术研究发展计划重大专项（2002AA206621）. |
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| 摘 要： | 利用精子载体法制备转基因动物是一种简便、可行的研究和应用方法，但精子结合并内化转运外源DNA却具有非常复杂的机制。从精子结合外源DNA的物质基础及分子机制，外源DNA在精子细胞中的存在和降解等方面展开探讨，并着重探讨了外源DNA在精子基因组中的整合机制。另外，基于理论研究的进展讨论了转基因动物的制备策略。
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| 关 键 词： | 转基因动物 精子载体 |
| 文章编号： | 1008-0864(2005)04-0038-03 |
| 收稿时间： | 03 15 2005 12:00AM |
| 修稿时间： | 06 15 2005 12:00AM |
Molecular Integration Mechanism of Sperm with Outer Origin DNA and Making About Transgenic Animals |
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| SUN Yu-jiang,WANG Li,LI Lan,PAN Qing-Jie.Molecular Integration Mechanism of Sperm with Outer Origin DNA and Making About Transgenic Animals[J].Journal of Agricultural Science and Technology,2005,7(4):38-40. |
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| Authors: | SUN Yu-jiang WANG Li LI Lan PAN Qing-Jie |
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| Institution: | 1. Dongying Institute of Agriculture Science, 2. Dongying Science Institute of Environment Protection, Shandong Dongying 257091, China|3. College of Animal Science and Technology, Laiyang Agriculture University, Shandong Laiyang 265200, China |
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| Abstract: | Making transgenic animal by SMGT is a very simple and feasible method for research and application, but combining and inner-transferring of outer origin DNA has very complicated mechanism, this article discussed about substance basic and molecular mechanism of integrate between sperm and outer origin DNA and probed into integrate mechanism of outer origin DNA in sperm genome. In addition, making method of transgenic animal was discussed on the basis of the development of theoretical research. |
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| Keywords: | transgenic animal sperm-mediated gene transfer |
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