Extracts of killedPenicillium chrysogenum Induce resistance against Fusarium wilt of melon |
| |
| Authors: | Hezhong Dong Yigal Cohen |
| |
| Institution: | (1) Bar-Ilan University, 52900 Ramat Gan, Israel;(2) On sabbatical leave from Cotton Research Center, Shandong Academy of Agricultural Sciences, 250100 Jinan, China |
| |
| Abstract: | Dry fungal biomass ofPenicillium chrysogenum (dry mycelium), a waste product of the pharmaceutical industry, was extracted with water and applied to the roots of melon
plants before or after inoculation withFusarium oxysporum f.sp.melonis (Font). Seedlings (4–6 days after emergence) treated with either acidic dry mycelium extract (DME) or neutralized dry mycelium extract
(NDME) were protected against challenge infection withFom. A single drench with 2–5% DME applied 12–72 h before inoculation provided significant control of the disease compared with
water-drenched, challenged seedlings. No protection was seen in plants treated 0–6 h before inoculation or 0–48 h after inoculation.
Neither DME nor NDME (0.5–5%) had any effect on fungal growthin vitro, which implied that disease controlin vivo was mediated by induced resistance. The resistance induced by DME protected melon plants not only against race 1,2, but also
against the three other races of the pathogen, indicating a race-non-specific resistance againstFom. Both DME and NDME significantly increased peroxidase activity and free L-proline content in seedlings 12 h and 48 h after
soil drench, respectively. Resistance to Fusarium wilt was significantly associated with elevated levels of peroxidase activity
but not with free L-proline content. Thus, peroxidase might be involved in the defense mechanisms activated by DME or NDME.
http://www.phytoparasitica.org posting Aug. 31, 2001. |
| |
| Keywords: | Penicillium chrysogenum Fusarium wilt Fusarium oxysporum f sp melonis induced resistance peroxidase L-proline |
| 本文献已被 SpringerLink 等数据库收录！ |
|
