| 16S rRNA鉴定乳酸片球菌分离株 |
| |
| 引用本文： | 韩烨,周志江,王培培,张丽霞.16S rRNA鉴定乳酸片球菌分离株[J].吉林农业大学学报,2008,30(3). |
| |
| 作者姓名： | 韩烨 周志江 王培培 张丽霞 |
| |
| 作者单位： | 1. 天津大学农业与生物工程学院,天津,300072
2. 天津大学化工学院,天津,300072 |
| |
| 摘 要： | 根据乳酸片球菌的16S rRNA基因序列,设计2条特异性引物,以PCR方法扩增从酸白菜中分离得到的具有抑制单核细胞增多症李氏杆菌作用的乳酸片球菌菌株的16S rRNA基因序列,经克隆和测序后,与以往发表的基因序列进行比较,同源性99%,从基因水平上进一步证明该菌株为乳酸片球菌。
|
| 关 键 词： | 16S rRNA PCR 乳酸片球菌 |
Identification of Pediococcus acidilactici by Using 16S rRNA Sequence Analysis |
| |
| HAN Ye,ZHOU Zhi-jiang,WANG Pei-pei,ZHANG Li-xia.Identification of Pediococcus acidilactici by Using 16S rRNA Sequence Analysis[J].Journal of Jilin Agricultural University,2008,30(3). |
| |
| Authors: | HAN Ye ZHOU Zhi-jiang WANG Pei-pei ZHANG Li-xia |
| |
| Abstract: | A pair of primers were designed based on the 16S rRNA gene sequence of a Pediococcus acidilactici strain.The 16S rRNA gene sequence was amplified by PCR from a Pediococcus acidilactici strain isolated from fermented Chinese cabbage,which had an inhibiting activity against Listeria monocytogenes.After cloning and sequencing this gene,the sequence homology was 99% of that of the published strain.This result suggested that the strain isolated in this study was Pediococcus acidilactici. |
| |
| Keywords: | 16S rRNA PCR Pediococcus acidilactici |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
