| 能量对初情期前母猪卵巢LHR和FSHR mRNA表达的影响 |
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| 引用本文： | 周虚,于淼瑛,刘立文,易康乐,李纯锦,陈璐,孙艳玲.能量对初情期前母猪卵巢LHR和FSHR mRNA表达的影响[J].中国兽医学报,2009,29(1). |
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| 作者姓名： | 周虚 于淼瑛 刘立文 易康乐 李纯锦 陈璐 孙艳玲 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林,长春,130062
2. 吉林大学畜牧兽医学院,吉林,长春,130062;河北北方学院,河北,张家口,075131 |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划 |
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| 摘 要： | 对9头50日龄、体质量为30 kg的初情期前杜×长×大三元杂交母猪进行长期日粮能量差异饲养试验.饲养结束后屠宰,取卵巢液氮保存.半定量RT-PCR检测每头母猪卵巢LH和FSH受体mRNA的含量.结果,高能组卵巢LH和FSH受体mRNA的含量显著高于中能组和低能组(P<0.05);而低能组显著低于中能组和高能组(P<0.05).表明,能量水平高的日粮可以显著地促进初情期前卵巢上LH和FSH受体在mRNA水平的表达,而能量水平不足的日粮则不利于这种表达.本文首次报道了日粮能量水平对母猪卵巢LH和FSH受体mRNA表达的影响.
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| 关 键 词： | LH、FSH受体 mRNA表达 初情期前母猪 |
Effects of dietary energy level on ovarian expression of mRNA s for luteinizing hormone receptor and follicle-stimulatiing hormone receptor in prepubertal gilts |
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| ZHOU Xu,YU Miao-ying,LIU Li-wen,YI Kang-le,LI Chun-jin,CHEN Lu,SUN Yan-ling.Effects of dietary energy level on ovarian expression of mRNA s for luteinizing hormone receptor and follicle-stimulatiing hormone receptor in prepubertal gilts[J].Chinese Journal of Veterinary Science,2009,29(1). |
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| Authors: | ZHOU Xu YU Miao-ying LIU Li-wen YI Kang-le LI Chun-jin CHEN Lu SUN Yan-ling |
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| Abstract: | To explore the effects of long-term treatment of different dietary energy levels on ovarian expression of mRNAs for LHR and FSHR,the present study was performed in nine growth-matched littermate crossbred (Land-race×Large White X Duroc) prepubertal gilts. At approximately 30 kg of body weight and 50 day of age,gilts were housed with individual feeding stalls and placed on a normal level of feeding for 90 days (dl-90) with free ac-cess to water and food throughout the whole research. From d91 ,littermates were divided and randomly assigned to one of the following three treatment lines for 3 weeks till d112:Group H,Group M, and Group L, fed the high energy level diet (n = 3, digestible energy 14.87 MJ/kg), moderate energy level diet (n = 3, digestible energy 12.39 MJ/ kg), and low energy level diet (n = 3, digestible energy 9.98 MJ/kg), respectively. When gilts were slaughtered on d112 after 3 weeks energy treatment, both ovaries of every gilts were collected,snap frozen in liquid nitrogen and re-tained at -80℃ for use to determine and analysis the relative amount of ovarian LHR and FSHR mRNAs using semi-quantitative RT-PCR. Results showed that the effect of dietary energy treatment was notable: the ovarian ex-pression of mRNAs for LHR and FSHR was significantly higher (P<0.05) in gilts treated with high energy diet compared to gilts treated with moderate and low energy diets, while gilts treated with low energy diet had a signifi-cantly lower (P<0.05) ovarian LHR and FSHR expression compared with gilts treated with moderate and high en-ergy diets. These results revealed that ovarian expression of I.HR and FSHR in prepubertal gilts increased as the lev-el of dietary energy intake elevated,i, e. , high energy diet can markedly enhance ovarian expression of mRNAs for LHR and FSHR,whereas energy deficit markedly suppress the expression. |
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| Keywords: | LH dietary energy LHR and FSHR mRNA expression prepubertal gilts |
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