| 龙葵SorMT2a和SorMT2c基因的克隆及其转化烟草研究 |
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| 引用本文： | 范仲学,蔡利娟,杜瑞雪,全先庆.龙葵SorMT2a和SorMT2c基因的克隆及其转化烟草研究[J].山东农业科学,2010(12):1-5. |
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| 作者姓名： | 范仲学 蔡利娟 杜瑞雪 全先庆 |
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| 作者单位： | 山东省农业科学院高新技术研究中心/山东省作物与畜禽品种改良生物技术重点实验室/农业部黄淮海作物遗传改良与生物技术重点开放实验室; |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金 |
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| 摘 要： | 以龙葵为试验材料,利用RT-PCR技术分离到2个长度不同的编码Ⅱ类MT（金属硫蛋白）的cDNA克隆SorMT2a（EU760481）和SorMT2c（EU760483）,成功构建植物表达载体pROKⅡ-SorMT2a和pROKⅡ-SorMT2c,通过农杆菌介导法转化烟草并通过PCR验证了基因的表达情况。与野生植株相比,转SorMT2a和SorMT2c基因烟草对镉的耐受性不同,有的转SorMT2a基因植株在MS培养基中的CdCl2浓度达到200μmol/L时仍然生长良好,生物量和株高均大于野生型植株;而转SorMT2c基因植株对镉的耐受性没有提高。这说明SorMT2a基因在植物对镉的耐受性中起作用,而SorMT2c基因则不起作用,从而也说明了同一类基因不同家族可能具有不同功能。
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| 关 键 词： | 龙葵 金属硫蛋白 重金属 RT-PCR SorMT2a SorMT2c |
Cloning of SorMT2a and SorMT2c from Solanum nigrum and Study on Their Transformation to Nicotiana tobacum |
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| FAN Zhong-xue,CAI Li-juan,DU Rui-xue,QUAN Xian-qing.Cloning of SorMT2a and SorMT2c from Solanum nigrum and Study on Their Transformation to Nicotiana tobacum[J].Shandong Agricultural Sciences,2010(12):1-5. |
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| Authors: | FAN Zhong-xue CAI Li-juan DU Rui-xue QUAN Xian-qing |
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| Institution: | FAN Zhong-xue,CAI Li-juan,DU Rui-xue,QUAN Xian-qing(High-Tech Research Center,Shandong Academy of Agricultural Sciences/ Key Laboratory for Genetic Improvement of Crop,Animal and Poultry of Shandong Province/ Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China) |
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| Abstract: | From Solanum nigrum L.,SorMT2a(EU760481)and SorMT2c(EU760483)with different number of nucleotides and encoding type Ⅱmetallothionein were isolated by RT-PCR.The plant expression vectors pROKⅡ-SorMT2a and pROKⅡ-SorMT2c were successfully constructed and transformed to the tobacco(Nicotiana tobacum) by Agrobacterium mediation and the transgenic seedlings were verified by PCR.The experiment on Cd tolerance indicated that transgenic plants of SorMT2a and SorMT2c had different tolerance to Cd.Compared with wild seedlings,most of the transgenic seedlings of SorMT2a could grow well in the MS medium supplemented with 200 μmol/L CdCl2 with larger biomass and greater plant height,while the Cd tolerance of transgenic seedlings of SorMT2c was not enhanced.From these results,the conclusion could be made that SorMT2a played a role in the Cd tolerance while SorMT2c did not have this function,which might suggest that different families of the MTs have different functions. |
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| Keywords: | Solanum nigrum Metallothionein Heavy metal RT-PCR SorMT2a SorMT2c |
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