| 禽呼肠孤病毒ZJS株种毒的纯净性鉴定 |
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| 引用本文： | 李慧姣.禽呼肠孤病毒ZJS株种毒的纯净性鉴定[J].中国兽药杂志,2011,45(3):23-25. |
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| 作者姓名： | 李慧姣 |
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| 作者单位： | 1. 中国兽医药品监察所,北京,100081
2. 广东大华农动物保健品股份有限公司,广东,新兴,527400 |
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| 基金项目： | 致谢：无菌检验和支原体检验项均由本所细菌制品检测室完成,在此,表示衷心的感谢! |
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| 摘 要： | 将禽呼肠孤病毒ZJS株细胞毒(弱毒)在鸡胚成纤维细胞(CEF)上连续传代,对其CI、C2、C3代种毒进行了无菌和支原体污染检验,对C5、C8和C10代种毒进行了外源病毒污染检验,对C3、C8和C10代种毒还进行了特异性鉴定,以确定禽呼肠孤病毒ZJS株细胞毒(弱毒)作为种毒的可行性.结果显示,禽呼肠孤病毒ZJS株细胞毒(...
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| 关 键 词： | 禽呼肠孤病毒 纯净性检验 特异性鉴定 |
| 收稿时间： | 2011/1/18 0:00:00 |
| 修稿时间： | 2011/1/20 0:00:00 |
Purity and Identity Test for Reovirus Seeds (Strain ZJS ) |
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| LIHUIJIAO.Purity and Identity Test for Reovirus Seeds (Strain ZJS )[J].Chinese Journal of Veterinary Drug,2011,45(3):23-25. |
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| Authors: | LIHUIJIAO |
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| Institution: | The institute of veterinary drug control |
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| Abstract: | Reovirus seeds (Strain ZJS ) were continuously passaged on primary CEF. Sterility and mycoplasmas test were done on passage 1, 2 and 3. Identity and extraneous test were taken on passage 5, 8 and 10. No bacteria, fungi, mycoplasmas and extraneous viruses can be found for all passages. The seed viruses were neutralized with antiserum of reovirus in the identity test. The results showed that Reovirus seeds (Strain ZJS ) complied with the purity and identity requirements for seed virus. |
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| Keywords: | Reovirus Identity test Purity test |
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