| 玉米矮花叶病毒HC-Pro的纯化及抗血清制备 |
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| 引用本文： | 李向东,范在丰,李怀方,裘维蕃.玉米矮花叶病毒HC-Pro的纯化及抗血清制备[J].植物病理学报,2001,31(2):135-138. |
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| 作者姓名： | 李向东 范在丰 李怀方 裘维蕃 |
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| 作者单位： | 中国农业大学植物保护学院植病系, 北京 100094 |
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| 基金项目： | 攀登计划(85-31-02-03) |
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| 摘 要： | 本实验提纯了玉米矮花叶病毒(MDMV)的辅助成份-蛋白酶(HC-Pro),并制备了其抗血清。MDMV HC-Pro的分子量约为57kD,提纯产量为0.3mg/100 g病叶。琼脂免疫双扩散测定证明MDMV HC-Pro抗血清的效价为1:16;微量免疫沉淀法测定时其效价为1:1024。该抗血清只与MDMV HC-Pro有明显的反应,与健康玉米汁液和MDMV无反应。MDMV HC-Pro抗血清可以专化性地抑制HC-Pro的蚜传活性。
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| 关 键 词： | 玉米 病毒病 玉米矮花叶病毒 辅助成份-蛋白酶 蚜虫传毒 |
| 文章编号： | 0412-0914(2001)02-0135-04 |
| 修稿时间： | 2000年4月15日 |
PURIFICATION AND ANTISERUM PREPARATION OF MAIZE DWARF MOSAIC VIRUS HC-Pro |
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| LI Xiang dong,FAN Zai feng,LI Huai fang,QIU Wei fan WTBZ.PURIFICATION AND ANTISERUM PREPARATION OF MAIZE DWARF MOSAIC VIRUS HC-Pro[J].Acta Phytopathologica Sinica,2001,31(2):135-138. |
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| Authors: | LI Xiang dong FAN Zai feng LI Huai fang QIU Wei fan [WTBZ] |
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| Institution: | Dept. of Plant Pathology, China Agricultural University, Beijing 100094, China |
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| Abstract: | Maize dwarf mosaic virus (MDMV) helper component proteinase (HC Pro) was purified and the specific antiserum to it was prepared in this experiment. The molecular weight of MDMV HC Pro was ca. 57 kD and its yield was 0.3 mg/100 g diseased leaves. The titer of antiserum was 1/16 in agar gel double diffusion test and 1/1024 in microprecipitation test. The antiserum reacted strongly with MDMV HC Pro, but did not with MDMV and healthy maize extracts. It could inhibit transmission efficiency of virus by aphids specifically. |
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| Keywords: | maize virus disease maize dwarf mosaic virus helper component proteinase aphid transmission |
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