| 甘薯无病毒苗培育技术研究 |
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| 引用本文： | 尚佑芬,杨崇良,赵玖华,李长松.甘薯无病毒苗培育技术研究[J].植物保护学报,1998,25(2):121-124. |
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| 作者姓名： | 尚佑芬 杨崇良 赵玖华 李长松 |
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| 作者单位： | 山东省农业科学院植物保护研究所，山东省农业科学院植物保护研究所，山东省农业科学院植物保护研究所，山东省农业科学院植物保护研究所 济南 250100，济南 250100，济南 250100，济南 250100 |
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| 摘 要： | １９９１￣１９９６年，甘薯茎尖离体培养采用ＭＳ为基本培养基，添加６－苄基氨基嘌呤（６－ＢＡ）０．５ｍｇ／Ｌ＋萘乙酸（ＮＡＡ）０．２ｍｇ／Ｌ＋腺素（Ａｄ）５ｍｇ／Ｌ，培育出１２个甘薯品种的脱毒苗，茎尖培养成苗率５０．０％￣９５．６％，成苗期４３￣６０ｄ。切取的茎尖大小与脱毒效果呈显著的负相关（ｒ＝－０．９８７５），茎尖带１个叶原基时，ＳＰＦＭＶ脱毒率为３８．１％，培养基中添加适量的植物病毒钝化剂可使
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| 关 键 词： | 甘薯 茎尖培养 脱毒 病毒检测 无病毒苗 培育 |
TECHNIQUES FOR VIRUS-FREE SWEET POTATO CULTURE |
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| Shang Youfen,Yang Chongliang,Zhao Jiuhua and Li Changsong.TECHNIQUES FOR VIRUS-FREE SWEET POTATO CULTURE[J].Acta Phytophylacica Sinica,1998,25(2):121-124. |
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| Authors: | Shang Youfen Yang Chongliang Zhao Jiuhua and Li Changsong |
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| Institution: | Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan,Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan,Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan and Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan |
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| Abstract: | 12 sweet potato cultivars free from virus were obtained by meristem tip culture during 1991 - 1996. The MS agar medium used contained 0. 5 mg/L of 6-BA, 0. 2 mg/L NAA and 5 mg/L Ad. The plantlet ratio was 50. 5%-95. 6% from meristem tip. The form of plantlet took 43-60 d. The size of meristem tip cut was negatively related to the percentage of virus-free plantlet obtained (r =-0. 9875). The addition of 0. 015 mg/L of TS in medium exhibited the inhibitory effect on virus multiplication. It, however, had negative effect to the ratio of plantlet. SPFMV, SPLV and TMV can be detected using Dot-ELISA with SPFMV monoclonal antibody and TMV antiserum in combination with I. setosa and C. amaranticolor as identification hosts. |
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| Keywords: | sweet potato meristem tip culture virus elimination detection virus |
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