| 3株O型FMDV VP1基因的克隆与序列分析 |
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| 引用本文： | 李浩波,马静云,李大旭,王玲玲,谢青梅,毕英佐.3株O型FMDV VP1基因的克隆与序列分析[J].动物医学进展,2008,29(4):26-30. |
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| 作者姓名： | 李浩波 马静云 李大旭 王玲玲 谢青梅 毕英佐 |
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| 作者单位： | 华南农业大学动物科学学院,广东广州,510642 |
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| 基金项目： | 广东省重大科技专项基金
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国家博士科研启动项目
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华南农业大学校科研和教改项目 |
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| 摘 要： | 以3株国内分离的O型口蹄疫病毒(FMDV)(分别命名F1、F2、F3)为研究目标,根据GenBank中注册的FMDV VP1基因的序列设计2对引物,采用RT-PCR方法成功地扩增出含有VP1全基因的cD-NA片段,将3个cDNA片段分别克隆到pMD20-T Vector载体中进行序列测定,得到3个毒株VP1基因的序列。结果表明,3个O型FMDV毒株VP1基因cDNA长度均为639 bp,编码213个氨基酸。3株O型毒株彼此之间的核苷酸序列同源性在92.3%~94.2%之间,推导氨基酸序列同源性在97.2%~98.6%之间。与3个毒株同源性高的主要为香港和台湾的毒株。
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| 关 键 词： | 口蹄疫病毒 VP1基因 序列分析 |
| 文章编号： | 1007-5038(2008)04-0026-05 |
| 修稿时间： | 2008年1月14日 |
Cloning and Sequence Analysis of the VP 1 Gene of Three Strains of Foot-and-mouth disease virus Type O |
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| LI Hao-bo,MA Jing-yun,LI Da-xu,WANG Ling-ling,XIE Qing-mei,BI Ying-zuo.Cloning and Sequence Analysis of the VP 1 Gene of Three Strains of Foot-and-mouth disease virus Type O[J].Progress In Veterinary Medicine,2008,29(4):26-30. |
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| Authors: | LI Hao-bo MA Jing-yun LI Da-xu WANG Ling-ling XIE Qing-mei BI Ying-zuo |
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| Abstract: | VP1 gene cDNA fragments of three strains of FMDV type O were amplified by RT-PCR respectively.PCR products were cloned and sequenced.The results suggested that the full-length of FMDV serotype O VP1 gene all comprised 639bp,coding for 213 amino acids.The nucleotide sequence homologies among 3 strains were between 92.3%-94.2%,and those of the amino acid sequence between 97.2%-98.6%.They were most closely related to strains isolated in Hong Kong and Taiwan. |
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| Keywords: | Foot-and-mouth disease virus VP1 gene sequence analysis |
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