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桑叶褐斑病病原菌的生物学特性研究
引用本文:吴庭观,章一鸣,叶政平,何永宏,张泽,徐升胜,洪逗.桑叶褐斑病病原菌的生物学特性研究[J].云南林业科技,2014(3):117-122.
作者姓名:吴庭观  章一鸣  叶政平  何永宏  张泽  徐升胜  洪逗
作者单位:[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南农业大学植保学院,云南昆明650201
基金项目:云南省农业厅项目“云南省现代农业蚕桑产业技术体系构建”.
摘    要:通过对桑叶褐斑病病原菌在不同种类培养基培养、不同氮源与碳源、不同温度等6种条件下培养6天后,测定菌落直径,观察菌丝颜色和菌落形态,分析了桑褐斑病病原菌的生物学特性。结果表明,在室内培养条件下,不同的营养成分、 pH值、温度、光照都对菌丝的生长有影响。病原菌菌丝生长的最适宜培养基是燕麦片培养基,适宜生长温度范围是20~30℃,最适生长温度为25℃,光照对菌丝生长无显著影响,最适pH值为9~10,最适碳源为α-乳糖,最适氮源为尿素,菌丝在55℃水浴10 min后则不能生长。

关 键 词:桑叶  褐斑病  病原菌  生物学特性

Bilolgical Characteristics of Pathogenic Fungus Causing Brown Spot on Mulberry Leaves
WU Ting-guan,ZHANG Yi-ming,YE Zheng-ping,HE Yong-hong,ZHANG Ze,XU Sheng-sheng,HONG Dou.Bilolgical Characteristics of Pathogenic Fungus Causing Brown Spot on Mulberry Leaves[J].Yunnan Forestry Science and Technology,2014(3):117-122.
Authors:WU Ting-guan  ZHANG Yi-ming  YE Zheng-ping  HE Yong-hong  ZHANG Ze  XU Sheng-sheng  HONG Dou
Institution:1. College of Horticulture and Landscape, Yunnan Agricultural University, Kunming Yunnan 650201, P. R. China; 2. College of Plant Protection , Yunnan Agricultural University, Kunming Yunnan 650201, P. R. China)
Abstract:Through cultivating pathogenic fungi of mulberry brown rot disease under 6 conditions such as different media, temperature, carbon source and nitrogen source after 6 days, research on the biological characteristics of pathogenic fungus causing brown spot on mulberry leaves was conducted .The results showed that nutrition , temperature and pH had significant influence on mycelial growth . It was found that the best medium for mycelial growth was oatmeal medium , the proper temperature was 20-30℃, the optimal temperature for mycelial growth was 25℃, the light had no obvious promotion effect on mycelial growth .It was also found that the optimal pH was 9 to 10 , the best carbon source and nitrogen source for mycelial grow are α-Galoctaose and Carbamide , and the mycelial could not survive above 55℃more than 10 min.
Keywords:Mulberry brown spot disease  pathogenic fungus  biological characteristics
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