| 棘胸蛙虹彩病毒免疫方法研究 |
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| 引用本文： | 胡金春,胡霭臻,金米雪,林威丞,谢梦佳,包伊鹏,李柯,郑善坚.棘胸蛙虹彩病毒免疫方法研究[J].安徽农业科学,2018,46(7):86-87,154. |
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| 作者姓名： | 胡金春 胡霭臻 金米雪 林威丞 谢梦佳 包伊鹏 李柯 郑善坚 |
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| 作者单位： | 浙江师范大学野生动物保护与利用技术中心,浙江金华321004;浙江省衢州市水产技术推广站,浙江衢州324002;浙江师范大学野生动物保护与利用技术中心,浙江金华,321004 |
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| 摘 要： | 目的]研究棘胸蛙虹彩病毒疫苗体表喷雾和肌肉注射的免疫效果,为其免疫方法提供理论依据。方法]用不同浓度棘胸蛙虹彩病毒灭活疫苗进行体表喷雾免疫和肌肉注射免疫,并进行血清抗体效价检测和相对免疫保护率测定。结果]注射0.2 m L 1×108TCID50/m L疫苗组21 d血清中和抗体效价最高达到380.2,相对免疫保护率达到80%;体表喷雾1×107TCID50/m L疫苗组,28 d最高抗体效价为320.0,相对免疫保护率为60%。结论]肌肉注射疫苗和体表喷雾疫苗对棘胸蛙都有较好的免疫效果,肌肉注射疫苗组的免疫效果最佳,但喷雾免疫可作为棘胸蛙快速无损伤免疫的一种有效途径。
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| 关 键 词： | 棘胸蛙 虹彩病毒 喷雾免疫 注射免疫 |
Study on the Immunological Method of Iridescent Virus in Quasipaa spinosa |
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| Abstract: | Objective]To determine the immune effect of body surface spray and intramuscular injection of iridescent virus vaccine onQ ua -sipaa spinosa, and provide theoretical basis for its immunization.Method]Q.spinosa was immunized with inactivated vaccine of iridescent virus by body surface spraying and muscle injection to determine serum antibody titer and relative immunity protection rate.Result]The highest titer of serum neutralizing antibody was 380.2 in 0.2 mL 1×108 TCID50/mL vaccine group after 21 days,and the relative immune pro-tection rate was 80%.In the body surface spraying group(1×107 TCID50/mL), the highest antibody titer was 320.0 after 28 days, and the relative immune protection rate was 60%.Conclusion]Intramuscular vaccine and body surface spray vaccine both had good immune effects on Q.spinosa,and the immunization effect of intramuscular injection group was better,but the body surface spray immunization could be used as an effective way for fast and non-injury immunization of Q.spinosa. |
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