| 变异冬红果无菌体系的建立 |
| |
| 引用本文： | 韦晨,王醒.变异冬红果无菌体系的建立[J].安徽农业科学,2018,46(10):92-93,122. |
| |
| 作者姓名： | 韦晨 王醒 |
| |
| 作者单位： | 江苏大学,现代农业装备与技术教育部重点实验室,江苏镇江212013;江苏艺轩园林景观工程有限公司,江苏镇江,212000 |
| |
| 基金项目： | 镇江市重点研发计划-现代农业(NY2015013)，江苏省农业科技自主创新资金项目(CX(15)1004) |
| |
| 摘 要： | 目的]建立变异冬红果初代培养无菌体系。方法]以变异冬红果茎段为外植体,采用3种不同的消毒方法,通过统计污染率、褐化死亡率和成活率,比较变异冬红果茎段较适宜的消毒方法。结果]用0.05%氯化汞消毒的最佳方式为氯化汞消毒20 min,污染率为17.78%,褐化死亡率为11.11%,成活率为71.11%;用0.1%氯化汞消毒的最佳方式为氯化汞消毒8 min,污染率为22.22%,褐化死亡率为20.00%,成活率为50.00%;用75%乙醇结合0.1%氯化汞消毒的最佳方式为75%乙醇30 s结合0.1%氯化汞10 min进行消毒,污染率为5.56%,褐化死亡率为55.56%,成活率为38.89%。结论]变异冬红果茎段最佳的消毒方式为0.05%氯化汞消毒20 min,污染率为17.78%,褐化死亡率为11.11%,成活率为71.11%。
|
| 关 键 词： | 变异冬红果 消毒处理 组织培养 |
The Establishment of Sterile System of Mutant Malus spectabilis |
| |
| WEI Chen,WANG Xing.The Establishment of Sterile System of Mutant Malus spectabilis[J].Journal of Anhui Agricultural Sciences,2018,46(10):92-93,122. |
| |
| Authors: | WEI Chen WANG Xing |
| |
| Abstract: | Objective] To establish the sterile system for the first generation of mutant Malus spectabilis.Method] With mutant Malus spectabilis stem section as explant,three disinfection methods were adopted.By comparing the pollution rate,the mortality of browning and the survival rate,the suitable method of disinfection was selected.Result] The optimal disinfection method with 0.05％ mercuric chloride solution was that the mercuric chloride solution was sterilized for 20 min,the contamination rate was 17.78％,the mortality rate of browning was 11.11％,the survival rate was 71.11％.The optimal disinfection method for mercury solution was disinfection of mercuric chloride solution for 8 min,the contamination rate was 22.22％,browning mortality rate was 20.00％,survival rate was 50.00％.The best disinfection method with 75％ alcohol and 0.1％ mercuric chloride solution was 75％ alcohol for 30 s and 0.1％ mercuric chloride solution for 10min,the contamination rate was 5.56％,the browning mortality rate was 55.56％ and the survival rate was 38.89％.Conclusion] The best method was 0.05％ mercuric chloride solution disinfection for 20 min,the pollution rate was 17.78％,browning mortality was 11.11％,survival rate was 71.11％. |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
