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基于线粒体标记的淮南麻黄鸡遗传结构及其遗传多样性分析
引用本文:黄胜海,贾晓旭,陆俊贤,唐修君,樊艳凤,葛庆联,高玉时.基于线粒体标记的淮南麻黄鸡遗传结构及其遗传多样性分析[J].安徽农业大学学报,2018,45(3):400-403.
作者姓名:黄胜海  贾晓旭  陆俊贤  唐修君  樊艳凤  葛庆联  高玉时
作者单位:江苏省家禽科学研究所,扬州,225125;江苏省家禽科学研究所,扬州,225125;江苏省家禽科学研究所,扬州,225125;江苏省家禽科学研究所,扬州,225125;江苏省家禽科学研究所,扬州,225125;江苏省家禽科学研究所,扬州,225125;江苏省家禽科学研究所,扬州,225125
基金项目:国家自然科学基金(31501917;31372277),江苏省农业科技自主创新资金(X(16)1006)
摘    要:通过探究淮南麻黄鸡的遗传多样性和遗传结构,为淮南麻黄鸡的种质资源保护和开发利用提供遗传背景资料。对淮南麻黄鸡线粒体基因组(mitochondrial genome,mt DNA)控制区全序列进行测序,并结合红色原鸡的序列进行生物信息学分析。淮南麻黄鸡mt DNA控制区全长1 231~1 232 bp,共发现26处单核苷酸变异位点,核苷酸多样性(Pi)为0.006 6,单倍型多样性(Hd)为0.917,平均核苷酸差异(K)为7.573。群体共包含14种单倍型,可以分为A、B、C和E 4个分支分别包括3、3、7和1种单倍型。淮南麻黄鸡在线粒体水平上有较高的多态性,并且来自多个不同的母系,有很好的开发潜力。

关 键 词:淮南麻黄鸡  线粒体DNA  控制区  系统发育关系
收稿时间:2017/9/28 0:00:00

The genetic diversity and population structure of Huainan yellow partridge chickens based on mitochondrial DNA marker
HUANG Shenghai,JIA Xiaoxu,LU Junxian,TANG Xiujun,FAN Yanfeng,GE Qinglian and GAO Yushi.The genetic diversity and population structure of Huainan yellow partridge chickens based on mitochondrial DNA marker[J].Journal of Anhui Agricultural University,2018,45(3):400-403.
Authors:HUANG Shenghai  JIA Xiaoxu  LU Junxian  TANG Xiujun  FAN Yanfeng  GE Qinglian and GAO Yushi
Institution:Jiangsu Institute of Poultry Science, Yangzhou 225125,Jiangsu Institute of Poultry Science, Yangzhou 225125,Jiangsu Institute of Poultry Science, Yangzhou 225125,Jiangsu Institute of Poultry Science, Yangzhou 225125,Jiangsu Institute of Poultry Science, Yangzhou 225125,Jiangsu Institute of Poultry Science, Yangzhou 225125 and Jiangsu Institute of Poultry Science, Yangzhou 225125
Abstract:This study was to investigate the genetic diversity and population structure of Huainan yellow partridge chickens. The complete D-loop region of mitochondrial DNA (mtDNA) of 32 chickens were sequenced and compared with published data of the red jungle fowl. The D-loop region of Huainan yellow partridge chickens was 1 231-1 232 bp in length. The average haplotype diversity, nucleotide diversity and nucleotide differences were 0.917, 0.006 6, and 7.573, respectively. A total of 14 haplotypes were identified, which belonged to four previously published clades, i.e. Clades A, B, C, and E, and contained 3, 3, 7, and 1 haplotypes, respectively. The results showed a high mitochondrial D-loop diversity and indicated multiple maternal origins of Huainan yellow partridge chickens. The results in this study will be a reference for conservation and utilization of Huainan yellow partridge chickens.
Keywords:Huainan yellow partridge chickens  mitochondrial DNA  D-loop region  phylogenetic relationship
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