| 几种因素对银杏毛状根诱导和黄酮含量的影响 |
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| 引用本文： | 邵菊芳,朱红威,张智,滕婷婷,邓焕平,杨海松,贺正旺.几种因素对银杏毛状根诱导和黄酮含量的影响[J].黑龙江农业科学,2008(6):10-12. |
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| 作者姓名： | 邵菊芳 朱红威 张智 滕婷婷 邓焕平 杨海松 贺正旺 |
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| 作者单位： | 中国矿业大学化工学院,徐州,221008 |
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| 基金项目： | 中国矿业大学校科研和教改项目，中国矿业大学校科研和教改项目 |
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| 摘 要： | 考察了发根农杆菌菌种、侵染时间、外植体类型、培养基类型和乙酰丁香酮5个因素对银杏毛状根诱导的影响，以及比较了后二因素对毛状根黄酮积累的影响。结果表明：以银杏胚为外植体，A4活化菌液中加入乙酰丁香酮100μmol·L^-1侵染15 min，再在MS培养基上培养发根率最高。乙酰丁香酮对毛状根中黄酮的积累没有显著促进作用，浓度高于120μmol·L^-1还会严重抑制发根。
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| 关 键 词： | 因素 银杏 毛状根 诱导 |
Effects of Several Factors on Hairy Root Inducement and Flavone Content of Ginkgo biloba |
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| SHAO Ju-fang,ZHU Hong-wei,ZHANG Zhi,TENG Ting-ting,DENG Huan-ping,YANG Hai-song,HE Zheng-wang.Effects of Several Factors on Hairy Root Inducement and Flavone Content of Ginkgo biloba[J].Heilongjiang Agricultural Science,2008(6):10-12. |
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| Authors: | SHAO Ju-fang ZHU Hong-wei ZHANG Zhi TENG Ting-ting DENG Huan-ping YANG Hai-song HE Zheng-wang |
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| Institution: | SHAO Ju-fang,ZHU Hong-wei,ZHANG Zhi,TENG Ting-ting,DENG Huan-ping,YANG Hai-song,HE Zheng-wang (Chemical Engineering , Technology College of China University of Mining , Technology,Xuzhou 221008) |
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| Abstract: | Five factors which influence inducement of Ginkgo biloba hairy roots, including Agrobacterium rhizogenes types,intrusion time,explant types, medium types and AS were reviewed in this article. The effect of the two later factors on flavones content in hairy roots was also compared. The results showed that：Rooting rates was the highest in the track that embryo intruded by activity A4 with 100 mol · L^-1 AS for 15 minutes and then cultured on MS medium. There was no markedly simulative function of AS for flavone accumulation in hairy roots. At the same time,rooting rate was badly restrained when AS content was higher than 120 mol·L^-1. |
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| Keywords: | factor Ginkgo biloba hairy root inducement |
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