| 四神叶形吸虫的DNA含量及蛋白质电泳分析 |
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| 引用本文： | 刘家英.四神叶形吸虫的DNA含量及蛋白质电泳分析[J].中国兽医寄生虫病,1996(1). |
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| 作者姓名： | 刘家英 |
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| 作者单位： | 华东师范大学生物学系 |
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| 摘 要： | 四种叶形吸虫成虫初级精母细胞核ＤＮＡ含量测定表明，寄生于较高等鱼类宿主的中华叶形吸虫和鳗鲡叶形吸虫的ＤＮＡ含量较高（Ｃ值分别为０．２５ｐｇ和０．２６ｐｇ），而寄生于较低等鱼类宿主的鲶叶形吸虫和巴氏叶形吸虫的ＤＮＡ含量较低（Ｃ值分别为０．２１ｐｇ和０．２０ｐｇ）。这四种叶形吸虫成虫全蛋白质浓度梯度凝胶电泳分析进一步显示，中华叶形吸虫与鳗鲡叶形吸虫构成一近缘亚群（Ｓｍ＝０．６５７９，Ｄ＝０．４１８７），而鲶叶形吸虫和巴氏叶形吸虫构成另一近缘亚群（Ｓｍ＝０．６１７６，Ｄ＝０．４８１９）；这两个亚群间的相似系数值（Ｓｍ）小于０．５３，遗传距离值（Ｄ）大于０．６４。以上结果从分子水平上再次证实将叶形吸虫属划分为两个亚属是有根据的。
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| 关 键 词： | 叶形属，DNA含量，蛋白质电泳 |
ON DNA CONTENTS AND PROTEIN PATTERNS OF FOUR SPECIES IN PHYLLODISTOMUM |
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| Liu Jiaying.ON DNA CONTENTS AND PROTEIN PATTERNS OF FOUR SPECIES IN PHYLLODISTOMUM[J].Chinese Journal of Veterinary Parasitology,1996(1). |
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| Authors: | Liu Jiaying |
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| Abstract: | The DNA contents of the four species in Phyllodistomum are measured withfluorescence microphotometry after Feulgan staining.P.sinense and P.anguilae have a higherDNA content(0.25 and 0.26 pg respectively);P.parasiluri and P.pawlovskii have a lowerDNA content(0.21 and 0.20 pg respectively).In the light of evolutionary theory it is suggested that the former two species are evolutionarily advanced than the latter two species.Thepatterns of general protein are analysed by means of gel concentration gradient electrophoresis. The result also indicates that P.sinense and P.anguilae constitute a related subgroup(Sm = 0.6579 and D = 0.4187);P.parasiluri and P.pewlovskii constitute another related subgroup(Sm = 0.6176 and D = 0.4819).There is a genetic difference between the two subgroup(Sm<0.53 and D>0.64) The present study provides the evidence at molecular level for dividing the genus Phyllodistomum into two subgenera. |
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| Keywords: | Phyllodistomum DNA content protein electrophoresis |
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