| 数字PCR在转基因定量检测中的研究进展 |
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| 引用本文： | 斯能武,李俊,武玉花,吴刚,张丽.数字PCR在转基因定量检测中的研究进展[J].中国油料作物学报,2021(1). |
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| 作者姓名： | 斯能武 李俊 武玉花 吴刚 张丽 |
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| 作者单位： | 中南民族大学生命科学学院/武陵山区特色资源植物种质保护与利用湖北省重点实验室;中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2016ZX08012-003);国家自然科学基金(31401607);国家自然科学基金(31601581)。 |
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| 摘 要： | 数字PCR对核酸分子进行定量检测时不依赖于标准曲线和标准物质,在转基因定量检测中可直接计算出模板中外源基因的拷贝数浓度值和转基因含量。本文概述了数字PCR在转基因定量检测中的应用,将数字PCR与实时荧光定量PCR的技术参数从检测特异性、线性动态范围、准确度、灵敏度、稳健性等方面进行了对比分析,以利其在转基因生物安全管理及转基因标识制度发挥技术支撑作用。本文还阐述了影响数字PCR检测结果的因素,探讨了数字PCR结果溯源至SI单位的可能性,为数字PCR在转基因定量检测以及其它领域核酸精确定量的应用提供参考。
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| 关 键 词： | 数字PCR 转基因生物 定量检测 绝对定量 |
Research progress of digital PCR in quantitative detection of genetically modified organism |
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| SI Neng-wu,LI Jun,WU Yu-hua,WU Gang,ZHANG Li.Research progress of digital PCR in quantitative detection of genetically modified organism[J].Chinese Journal of Oil Crop Sciences,2021(1). |
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| Authors: | SI Neng-wu LI Jun WU Yu-hua WU Gang ZHANG Li |
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| Institution: | (College of Life Science,South-Central University for Nationalities,Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China,Wuhan 430074,China;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China) |
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| Abstract: | Labeling of genetically modified organisms(GMOs)is an important part of safety management of GMOs.Quantitative detection technology provides important technical support for effective implementation of the GM labeling system.Digital PCR does not rely on standard curves and reference materials when performing quantitative detection of nucleic acid molecules,it could directly calculate copy number concentration of the detection target in sample.Its application in accurate quantification of nucleic acids is becoming more and more extensive.In the quantitative detection of GMOs,digital PCR could be used directly to calculate content of exogenous gene in template.By comparing of digital PCR and real-time fluorescent quantitative PCR in GMOs detection,factors affecting digital PCR detection results were elaborated,and the possibility of digital PCR results traceable to SI units was discussed.This study was expected to provide a reference for application of digital PCR in quantitative detection of GMOs and precise quantification of nucleic acids in several fields. |
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| Keywords: | digital PCR genetically modified organism quantitative detection absolute quantification |
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