| 油菜核盘菌对戊唑醇的敏感性及室内抗药性诱导 |
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| 引用本文： | 杨永青,宋培玲,燕孟娇,郝丽芬,连海飞,李秀萍,莎娜,史志丹,赵丽丽,李子钦.油菜核盘菌对戊唑醇的敏感性及室内抗药性诱导[J].中国油料作物学报,2021,43(2):300. |
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| 作者姓名： | 杨永青 宋培玲 燕孟娇 郝丽芬 连海飞 李秀萍 莎娜 史志丹 赵丽丽 李子钦 |
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| 作者单位： | 1. 内蒙古自治区农牧业科学院，内蒙古呼和浩特，010031；
2. 内蒙古大学生命科学学院，内蒙古呼和浩特，010021 |
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| 基金项目： | 内蒙古自治区农牧业科学院青年创新基金（2017QNJJNO4）；内蒙古自治区农牧业科学院青年创新基金（2018QNJJNO6） |
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| 摘 要： | 为了探明油菜核盘菌对戊唑醇的敏感性，对68株油菜核盘菌的戊唑醇室内敏感性进行了试验。同时通
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| 关 键 词： | 油菜核盘菌 戊唑醇 室内敏感性 抗药性诱导 碱基突变 |
Sensitivity of Sclerotinia sclerotiorum to tebuconazole and its resistance induction in vitro |
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| YANG Yong-qing,SONG Pei-ling,YAN Meng-jiao,HAO Li-fang,LIAN Hai-fei,LI Xiu-ping,SHA Na,SHI Zhi-dan,ZHAO Li-li,LI Zi-qin.Sensitivity of Sclerotinia sclerotiorum to tebuconazole and its resistance induction in vitro[J].Chinese Journal of Oil Crop Sciences,2021,43(2):300. |
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| Authors: | YANG Yong-qing SONG Pei-ling YAN Meng-jiao HAO Li-fang LIAN Hai-fei LI Xiu-ping SHA Na SHI Zhi-dan ZHAO Li-li LI Zi-qin |
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| Institution: | 1. Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China;
2. Inner Mongolia University, Hohhot 010021, China |
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| Abstract: | To investigate sensitivity of Sclerotinia sclerotiorum to tebuconazole, 68 isolates of S. sclerotiorum
collected from Anhui, Hunan, Hubei, and Sichuan provinces of China in 2016 and 2018 were studied and resistant
mutants were induced with ultraviolet. Moreover, mycelium growth rate, sclerotia formation and sequences of the tar?
get gene CYP51 were tested and compared among mutants and wild type, aiming to provide basic understanding of
tebuconazole application and resistance risk. Results showed that the EC50 values (effective concentrations causing
50% mycelial growth inhibition) ranged from 0.405 to 2.942 μg/mL. Besides, the proportion of isolates with EC50 val?
ues between 1.0 to1.8 μg/mL was 73.5% and frequency distribution of EC50 values was unimodal, indicating the S.
sclerotiorum tested were sensitive to tebuconazole. Four tebuconazole resistant mutants of S. sclerotiorum were ob?
tained by ultraviolet-induction combined with culturing on PDA plates amended with tebuconazole. Resistance fac?
tors (RF) of induced mutants were 2.6, 2.9, 3.1, and 4.7, respectively. The resistant mutants exhibited significant fit?
ness penalties based on slower mycelium growth and less production of sclerotia compared to wild type，and a point
mutation, lysine to glutamic acid (K244E) within the target enzyme CYP51, was detected and associated with resis?
tance to tebuconazole, suggesting that tebuconazole resistance conferred by K244E mutation might not developed in
field epidemically. |
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| Keywords: | Sclerotinia sclerotiorum tebuconazole sensitivity in vitro induced resistance base mutation |
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