| 渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文) |
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| 引用本文： | 孙朝霞,侯思宇,王玉国.渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文)[J].农业科学与技术,2008,9(3):46-48. |
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| 作者姓名： | 孙朝霞 侯思宇 王玉国 |
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| 作者单位： | 山西农业大学农学院生物技术系;中国农业科学院生物技术研究所 |
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| 摘 要： | Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat.
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| 关 键 词： | Wheat Somatic embryogenesis Organic appendix Osmosis-regulating substance |
Study on the Effect of Osmosis-regulating Substances and Organic Appendices on Somatic Embryogenesis in Wheat |
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| SUN Zhao-xia,HOU Si-yu,WANG Yu-guo.Study on the Effect of Osmosis-regulating Substances and Organic Appendices on Somatic Embryogenesis in Wheat[J].Agricultural Science ＆ Technology,2008,9(3):46-48. |
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| Authors: | SUN Zhao-xia HOU Si-yu WANG Yu-guo |
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| Institution: | 1. Biotechnology Department of Agricultural College,Shanxi Agricultural University, Taigu 030801;2. Biotechnology Research Institute of Chinese Academy of Agriculltural Science,Beijing 100081 |
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| Abstract: | Objective] The study aimed to reveal the effect of osmosis-regulating substances and organic appendices on somatic embryogenesis in wheat. Method] The suitable concentration combination of appendices was optimized by adding different concentrations of osmosis-regulating substances including mannitol, sorbitol and organic appendices such as Gln, CH and LH, into the somatic embryogenesis in wheat. Result] The mannitol or sorbitol lower than 40 g/L was helpful for improving somatic embryogenesis; there was no significant difference in the induction rate of somatic embryogenesis when 300-500 mg/L Gln、CH or LH was respectively added into the induced medium, while somatic embryogenesis could be enhanced dramatically in the presence of 500 mg/L Gln together with 300 mg/L CH. Conclusion] Somatic embryogenesis could be improved to some extent by different concentrations of osmosis-regulating substances and organic appendices, which laid foundation for establishing a more perfect system of somatic embryogenesis in wheat. |
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| Keywords: | Wheat Somatic embryogenesis Organic appendix Osmosis-regulating substanceWANG Yu-guo Corresponding author E-mail: tgwygn@126 com |
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