Callus Formation and Plant Regeneration from Protoplasts Derived from Suspension Culture of Rice (Oryza sativa L. cv. 'Taipei 177') |
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| Authors: | G C Ghosh Biswas F J Zapata |
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| Institution: | G. C. Ghosh;Biswas, F. J. Zapata, The International Rice Research Institute, P.O. Box 933, Manila (Philippines) |
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| Abstract: | Protoplasts were isolated from young inflorescence-derived suspension cultures of a japonica rice cultivar ‘Taipei 177’. The isolated protoplasts which were cultured either in liquid, agar on Sea plaque agarose underwent sustained division. Maximum plating efficiency of 1.06% occurred in a medium containing macroelements of KM, microelements and vitamins of B5, 0.5 % Sea plaque agarose, 1.0 mg/l of 2,4-D, and glucose as an osmotic stabilizer. Green and albino plants were regenerated from the protocalli in MS semisolid medium containing 4 mg/l BAP, 0.5 mg/l NAA and 500 mg/l casein hydrolysate (MS18–2). |
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| Keywords: | Oryza sativa L protoplasts callus formation plant regeneration |
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