| 鸡法氏囊病野毒的分离,细胞毒的培养及鉴定 |
| |
| 引用本文： | 肖治军,胡毓骥.鸡法氏囊病野毒的分离,细胞毒的培养及鉴定[J].河南农业科学,1995(10):33-35. |
| |
| 作者姓名： | 肖治军 胡毓骥 |
| |
| 作者单位： | 河南省农业科学院畜牧兽医研究所，河南省农业科学院畜牧兽医研究所，河南省农业科学院畜牧兽医研究所，河南省农业科学院畜牧兽医研究所，河南省农业科学院畜牧兽医研究所 郑州 450002，郑州 450002，郑州 450002，郑州 450002，郑州 450002 |
| |
| 摘 要： | 从发生法氏囊病（ＩＢＤ）的免疫肉鸡群的法氏囊病料中分离到１株病毒。该病毒致使非免疫鸡胚病变、死亡和鸡胚成纤维细胞病变。将其５代内细胞培养毒回归非免疫鸡胚和适龄鸡，复制出类似于野毒引起的鸡胚病变和典型ＩＢＤ病例，鸡的死亡率为１／３￣２／３。其５代细胞毒的灭活制剂，使非免疫鸡在接种后２１天或３２天１００％出现ＩＢＤ琼扩阳性。
|
| 关 键 词： | 鸡法氏囊病 野毒 分离 细胞培养 鉴定 鸡病 IBD |
Isoltation of Field IBD Virus from Chicken and Cultivation and Primary Identification of the Cytoadaptive Virus |
| |
| Xiao Zhijun Hu Yuji Liu Wenhui Cao Chunjing Xu Feng.Isoltation of Field IBD Virus from Chicken and Cultivation and Primary Identification of the Cytoadaptive Virus[J].Journal of Henan Agricultural Sciences,1995(10):33-35. |
| |
| Authors: | Xiao Zhijun Hu Yuji Liu Wenhui Cao Chunjing Xu Feng |
| |
| Abstract: | A strain of field virus was isolated from the IBD broilers that had been vacci-nated twice with the IBD vaccine. The field virus caused lesions and death of the chick-en embryos and cytopathic effect of the chicken embryo fibroblasts. The cytoadaptive virus within 5 passages caused the same lesions of the chicken embryos and typical IBD cases with mortalities from 33. 3% to 66. 7%. The inactivated cytoadaptive vitus caused 100% positive serum antibodies of IBD AGP in the experimental chickens 21 or 32 days postinoculation. |
| |
| Keywords: | IBD field virus Isolation Cell culture Primary identification |
| 本文献已被 CNKI 维普 等数据库收录！ |
