| 高效液相色谱法分离纯化小皱蝽抗菌肽AMP-1 |
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| 引用本文： | 杨泽宏,杨星勇,袁小红,罗克明,罗小英,张永军,郭余龙,裴炎.高效液相色谱法分离纯化小皱蝽抗菌肽AMP-1[J].西南农业大学学报,2001,23(2):104-107. |
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| 作者姓名： | 杨泽宏 杨星勇 袁小红 罗克明 罗小英 张永军 郭余龙 裴炎 |
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| 作者单位： | 西南农业大学生物技术中心，重庆400716 |
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| 基金项目： | 重庆市科委攻关项目及西南农业大学专项基金项目 |
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| 摘 要： | 用昆虫生理盐水浸提小皱蝽成虫，浸提液冻干后，酸提取法制备粗提液，固相萃取（SPE）初分级分离，活性洗脱组分反相高效液相色谱（RP－HPLC）纯化，进一步凝胶渗透色谱（GPS）及反相高效液相反谱（RP－HPLC）纯化，分离得到一抗菌肽，茚三酮反应黄色，命名为AMP－1。在190nm-225nm短波紫外区，AMP－1有紫外吸收。
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| 关 键 词： | 小皱蝽 抗菌肽 纯化 HPLC |
| 文章编号： | 1000-26422(2001)02-0104-04 |
| 修稿时间： | 2000年10月20 |
PURIFICATION OF THE ANTIMICROBIAL PEPTIDE AMP- 1 OF CYCLOPELTA PARVA DISTANT BY HPIC |
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| YANG Ze-hong,YANG Xing-yong ,YUAN Xiao-hong,LUO Ke-ming LUO Xiao-ying,ZHANG Yong-jun,GUO Yu-long,PEI Yan.PURIFICATION OF THE ANTIMICROBIAL PEPTIDE AMP- 1 OF CYCLOPELTA PARVA DISTANT BY HPIC[J].Journal of Southwest Agricultural University,2001,23(2):104-107. |
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| Authors: | YANG Ze-hong YANG Xing-yong YUAN Xiao-hong LUO Ke-ming LUO Xiao-ying ZHANG Yong-jun GUO Yu-long PEI Yan |
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| Institution: | YANG Ze-hong,YANG Xing-yong *,YUAN Xiao-hong,LUO Ke-ming LUO Xiao-ying,ZHANG Yong-jun,GUO Yu-long,PEI Yan |
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| Abstract: | An antimicrobial peptide,AMP-1,was isolated from the bug Cyclopelta parva Distant,an insect of Pentatomidae,Hemiptera,by the extraction and preparation of the crude extract with the acid extraction methods and fractionation of Sep-Pak C 18 Cartridge.Subsequently,40% Sep-Pak fraction was purified by the initial reverse phase HPLC.The active peak was further fractionated by gel-permeation chromatography HPLC and the final reverse phase HPLC.All the HPLC purifications utilized the Acetonitrile-Water-Trifluoroacetic acetic elution system as mobile phase,with detection at 225 nm.The pure substances were recovered and the anti-bacterial activity of each aliquot was routinely monitored by the plate growth inhibition assay on S.aureus and E.coli, AMP-1 is the antimicrobial polypeptide. |
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| Keywords: | HPLC purification Cyclopelta parva Distant antimicrobial peptide |
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