| 牛分技杆菌胞浆特异蛋白质抗原的制备和检测 |
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| 引用本文： | 汪广荫
,林范泽
,卫广森
,曹殿军
,杨光.牛分技杆菌胞浆特异蛋白质抗原的制备和检测[J].黑龙江八一农垦大学学报,1990(1). |
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| 作者姓名： | 汪广荫 林范泽 卫广森 曹殿军 杨光 |
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| 摘 要： | 将牛分枝杆菌Mycobacterium boyis S_(10)株培养于苏通培养基,经低温高速离心,超声波裂解,Sephadex G200柱层析,获得3个峰,制备到3种胞浆蛋白质抗原,经同奶牛结核病分枝杆菌阳性血清。阴性血清及各种分枝杆菌高免牛血清的ELISA检测,并与牛PPD,聚合OT等抗原做了比较,以第二峰的胞浆蛋白质抗原的特异性为最好。
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| 关 键 词： | 牛分枝杆菌 抗原 血清 蛋白质 |
PREPARATION AND DETECTION OF SPECIFIC CYTOPLASM PROTEIN ANTIGEN OF MYCOBACTERIUM BOVIS |
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| Wang Guangyin Piao Fanze Wei Guangsen Cao Dianjuan Yang Gwang..PREPARATION AND DETECTION OF SPECIFIC CYTOPLASM PROTEIN ANTIGEN OF MYCOBACTERIUM BOVIS[J].Journal of Heilongjiang August First Land Reclamation University,1990(1). |
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| Authors: | Wang Guangyin Piao Fanze Wei Guangsen Cao Dianjuan Yang Gwang |
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| Institution: | Wang Guangyin Piao Fanze Wei Guangsen Cao Dianjuan Yang Gwang. |
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| Abstract: | Mycobacterlum bovis S10 cultured on soton's medium was processed by low temperature ultracentrifugation, ultrasonication. Sephadex G200 chromatography of preparation showed three peaks. Three kinds of protein antigen of the cytoplasm was obtained. According to decting the positive cows serum and negative cows serum of Mycobacterium bovis, and the cows nhyperimmune serum of various Myco bacterium by ELISA method, and in comparison with the cow PPD antigen and the cow polymerized OT antigen Specifiction of second peak of cytoplasm protein antigen was the best. |
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| Keywords: | Mycobacteriuta bovis Antigen Selum Protein |
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