| 初探lncRNA-Tubb4b的蛋白编码能力及其对微管基因的调控 |
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| 引用本文： | 冯美莹,卫恒习,李莉,张守全.初探lncRNA-Tubb4b的蛋白编码能力及其对微管基因的调控[J].畜牧兽医学报,2022,53(1):179-187. |
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| 作者姓名： | 冯美莹 卫恒习 李莉 张守全 |
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| 作者单位： | 1. 肇庆学院生命科学学院, 肇庆 526061;2. 华南农业大学动物科学学院 国家生猪种业工程技术研究中心 广东省农业动物基因组学与分子育种重点实验室, 广州 510642 |
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| 基金项目： | 广东省普通高校青年创新人才类项目(2018KQNCX293);肇庆学院博士启动项目(221807);肇庆学院实践教学改革研究项目(sjjx201910)。 |
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| 摘 要： | 旨在探索长链非编码Tubb4b基因(lncRNA-Tubb4b)是否具有蛋白编码能力及其对Tubb4b基因的调控作用.本研究在lncRNA-Tubb4b中添加起始密码子ATG、0/1/2个的T尾和Flag标记,通过构建载体、脂质体转染和Western blot等技术对lncRNA-Tubb4b蛋白编码能力进行分析;随后...
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| 关 键 词： | lncRNA-Tubb4b 微管 Tubb4b 蛋白编码 小鼠 |
| 收稿时间： | 2021-05-28 |
The Preliminary Research of the Protein Coding Ability of lncRNA-Tubb4b and Its Regulation on Microtubule Genes |
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| FENG Meiying,WEI Hengxi,LI Li,ZHANG Shouquan.The Preliminary Research of the Protein Coding Ability of lncRNA-Tubb4b and Its Regulation on Microtubule Genes[J].Acta Veterinaria et Zootechnica Sinica,2022,53(1):179-187. |
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| Authors: | FENG Meiying WEI Hengxi LI Li ZHANG Shouquan |
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| Institution: | 1. College of Life Science, Zhaoqing University, Zhaoqing 526061, China;2. Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | The study aimed to explore whether the long non-coding RNA of Tubb4b(lncRNA-Tubb4b)has protein coding capability and the regulatory effect of lncRNA-Tubb4b on the Tubb4b gene.The ATG,0/1/2 T tails and Flag sequence were added to lncRNA-Tubb4b to analyze the coding ability of lncRNA-Tubb4b by constructing vector,liposome transfection and Western blot;Then,overexpression vector of lncRNA-Tubb4b was constructed and the siRNA of lncRNA-Tubb4b was synthesized,while the liposome transfection and real-time fluorescent quantitative PCR technology were used to detect the influence of lncRNA-Tubb4b on the Tubb4b gene.The results showed that the Flag fragments with 0,1,and 2 T-tail,was amplified in this study,were fused with lncRNA-Tubb4b,then were successfully constructed into the pcDNA3.1(-)vector;After transfection of mouse spermatogonia,it was found that lncRNA-Tubb4b with 0,1,and 2 T-tail had no FLAG protein bands.With pcDNA 3.1(-)-EGFP vector as a control,overexpression of lncRNA-Tubb4b significantly reduced the mRNA expression level of Tubb4b in mouse spermatogonia(P<0.01),while there was fluorescence in control cells.And interfering the expression of lncRNA-Tubb4b significantly increased the mRNA expression level of Tubb4b in mouse spermatogonia(P<0.01).It is indicated that lncRNA-Tubb4b does not encode protein,and overexpression or interference with the expression of lncRNA-Tubb4b will extremely significantly reduce or increase the expression of Tubb4b. |
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| Keywords: | lncRNA-Tubb4b tubule Tubb4b protein coding mouse |
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