| N-乙酰半胱氨酸对山羊子宫内膜基质细胞增殖及相关基因表达的影响 |
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| 引用本文： | 杨沛方,陈祥,周志楠,唐文,张艳,惠茂茂.N-乙酰半胱氨酸对山羊子宫内膜基质细胞增殖及相关基因表达的影响[J].畜牧兽医学报,2022,53(1):141-150. |
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| 作者姓名： | 杨沛方 陈祥 周志楠 唐文 张艳 惠茂茂 |
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| 作者单位： | 1. 贵州大学高原山地动物遗传育种与繁殖教育部重点实验室 贵州省动物遗传育种与繁殖重点实验室, 贵阳 550025;2. 贵州大学动物科学学院, 贵阳 550025 |
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| 基金项目： | 国家自然科学基金(32060753);国家重点研发计划项目(2017YFD0501904);贵州省科技支撑计划项目(黔科合支撑[2021]一般151);贵州省肉羊产业技术体系项目(GZRYTX2019-01)。 |
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| 摘 要： | 旨在探究N-乙酰半胱氨酸(N-acetyl-L-cysteine,NAC)对山羊子宫内膜基质细胞(goat endometrial stro-mal cells,gESCs)的影响.本研究以山羊子宫内膜基质细胞为对象,体外培养基中添加浓度梯度分别为100、200、400μmol·L-1的NAC,将0μmol·L1 NA...
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| 关 键 词： | N-乙酰半胱氨酸 gESCs 细胞增殖 基因表达 繁殖性能 |
| 收稿时间： | 2021-04-30 |
The Effect of N-acetylcysteine on Proliferation and Related Gene Expression of Goat Endometrial Stromal Cells in vitro |
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| YANG Peifang,CHEN Xiang,ZHOU Zhinan,TANG Wen,ZHANG Yan,HUI Maomao.The Effect of N-acetylcysteine on Proliferation and Related Gene Expression of Goat Endometrial Stromal Cells in vitro[J].Acta Veterinaria et Zootechnica Sinica,2022,53(1):141-150. |
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| Authors: | YANG Peifang CHEN Xiang ZHOU Zhinan TANG Wen ZHANG Yan HUI Maomao |
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| Institution: | 1. Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Key Laboratory of Animal Genetics, Breeding and Reproduction in Plateau Mountainous Region, Ministry of Education, Guiyang 550025, China;2. College of Animal Science, Guizhou University, Guiyang 550025, China |
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| Abstract: | The aim was to explore the effect of N-acetyl-L-cysteine(NAC)on goat endometrial stromal cells(gESCs).In this study,goat endometrial stromal cells were used as the object.Three concentration gradients of 100,200,400μmol·L-1NAC were added to the culture medium.The 0μmol·L-1NAC was regarded as the blank group and CCK-8 method was used to screen the optimal concentration of NAC and judge its effect on cell proliferation.Then DCFH-DA probe and RT-qPCR were used to detect the optimal concentration of NAC on the intracellular reactive oxygen content,cell cycle and proliferation related factors,and goat reproductive performance-related genes TGF-β1 and TGF-β3 mRNA expression levels.The results showed that the concentration of 200μmol·L-1NAC had a more significant effect on the proliferation of goat endometrial stromal cells than other gradients.In addition,200μmol·L-1NAC reduced the ROS content of cells compared with the blank group(P<0.01),thereby delaying cell senescence.RT-qPCR results showed that the addition of 200μmol·L-1NAC significantly increased the expression levels of cell cycle and proliferation-related factors CyclinA1,CyclinE,PCNA mRNA(P<0.01),and reduced the mRNA expression of cell cycle factor CyclinD2(P<0.01);and extremely significantly reduced the TGF-β1 gene(P<0.01)of the transforming growth factor family and significantly reduced the mRNA expression level of the TGF-β3 gene(P<0.05).Therefore,this study speculates that 200μmol·L-1NAC may promote cell proliferation by regulating the expression levels of CyclinA1,CyclinE,PCNA,and CyclinD2 in cells,and affect goat reproduction traits by regulating the expression levels of transforming growth family factors TGF-β1 and TGF-β3.This study provide a theoretical basis for further research on the mechanism of NAC’s influence on goat reproductive traits. |
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| Keywords: | N-acetylcysteine gESCs cell proliferation gene expression reproductive performance |
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