| 新疆地区3株驴源马链球菌马亚种新SeM基因型的鉴定与进化分析 |
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| 引用本文： | 杜宇,蒲小峰,陈晓萌,吕芬芬,汪丽,张泽华,张宝江,苏艳.新疆地区3株驴源马链球菌马亚种新SeM基因型的鉴定与进化分析[J].畜牧兽医学报,2022,53(1):231-240. |
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| 作者姓名： | 杜宇 蒲小峰 陈晓萌 吕芬芬 汪丽 张泽华 张宝江 苏艳 |
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| 作者单位： | 新疆农业大学动物医学学院, 乌鲁木齐 830052 |
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| 基金项目： | 国家自然科学基金项目(U1803108)。 |
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| 摘 要： | 从新疆地区某驴养殖场获得了3株驴腺疫链球菌分离株HTP133、HTP123和HTP232.为了解这3株驴腺疫链球菌的生物学特性和确定其分子分型,本研究对其进行了生化特性、药敏特性的检测,对16S rRNA进行序列对比分析,并利用PCR扩增SeM等位基因和测序鉴定其基因型.研究结果表明3株分离菌均为马链球菌马亚种.药敏试...
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| 关 键 词： | 驴源马链球菌马亚种 16S rRNA分析 SeM分子分型 药敏特性 |
| 收稿时间： | 2021-04-07 |
Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp.equi Isolates in Xinjiang |
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| DU Yu,PU Xiaofeng,CHEN Xiaomeng,Lü Fenfen,WANG Li,ZHANG Zehua,ZHANG Baojiang,SU Yan.Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp.equi Isolates in Xinjiang[J].Acta Veterinaria et Zootechnica Sinica,2022,53(1):231-240. |
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| Authors: | DU Yu PU Xiaofeng CHEN Xiaomeng Lü Fenfen WANG Li ZHANG Zehua ZHANG Baojiang SU Yan |
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| Institution: | College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | Three isolates of Streptococcus subsp.equi HTP133,HTP123 and HTP232 were obtained from a donkey farm in Xinjiang.To identify the genotypingand understand the biological and epidemic characteristics of these three isolates,the biochemical characteristics analysis,drug sensitivity test and 16S rRNA sequence comparison analysis and phylogenetic tree construction were performed.The SeM alleles were amplified by PCR and sequenced for genotyping.The results showed that these three isolates were Streptococcus equi subsp.equi.These three isolates were sensitive to 18 drugs,including amoxicillin,ampicillin and gentamicin etc,whereas they are resistant to penicillin and cefuroxime.Form the 16S rRNA gene analysis and the phylogentic tree,we found that these isolated strains belonged to group I and there is an obvious variation in their variable regions of V1-V5.The homology of these three isolates are closely related with S.equi JQ726493.In addition,two new SeM genotypes,SeM 210(HTP232)and SeM 211(HTP133,HTP123)were identified by SeM allele analysis and they were closely related to SeM 136,SeM 138,SeM 142 and SeM 146 genotype.This work enriches the molecular biological foundation of prevalence and transmission characteristics of donkey S.equi in China and provides the theoreitical basis for the prevention and control of donkey strangles as well. |
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| Keywords: | donkey Streptococcus equi subsp equi 16S rRNA analysis SeM genotyping drug sensitivity |
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