Genetic relationships among Chickpea (<Emphasis Type="Italic">Cicer arietinum</Emphasis> L<Emphasis Type="Italic">.</Emphasis>) genotypes based on the SSRs at the quantitative trait Loci for resistance to Ascochyta Blight |
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| Authors: | B Tar’an T Warkentin A Tullu A Vandenberg |
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| Institution: | (1) Crop Development Centre, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, Canada, S7N 5A8 |
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| Abstract: | Breeding for resistance to ascochyta blight in chickpea has been challenged by several factors including the limited sources
of good resistance. Characterization of a set of genotypes that may contain different genes for resistance may help breeders
to develop better and more durable resistance compared to current cultivars. The objective of this study was to evaluate the
genetic relationships of 37 chickpea germplasm accessions differing in reaction to ascochyta blight using Simple Sequence
Repeat (SSR) markers linked to Quantitative Trait Loci (QTL) for resistance. The results demonstrated that ILC72 and ILC3279,
landraces from the former Soviet Union, had SSR alleles that were common among the kabuli breeding lines and cultivars. A
lower SSR allele diversity was found on LG4 than on other regions. No correlation was found between the dendrogram derived
using SSRs at the QTL regions and the SSRs derived from other parts of the genome. The clustering based on 127 alleles of
17 SSRs associated with the QTL for ascochyta blight resistance enabled us to differentiate three major groups within the
current germplasm accessions. The first group was the desi germplasm originating from India and cultivars derived from it.
The second group was a mix of desi genotypes originating from India and Greece, and kabuli breeding lines from ICARDA and
the University of Saskatchewan. The third and largest group consisted of landraces originating mostly from the former Soviet
Union and breeding lines/cultivars of the kabuli type. Several moderately resistance genotypes that are distantly related
were identified. Disease evaluation on three test populations suggested that it is possible to enhance the level of resistance
by crossing moderately resistant parents with distinct genetic backgrounds at the QTL for resistance to ascochyta blight. |
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| Keywords: | Chickpea Ascochyta blight resistance Quantitative Trait Loci (QTL) Simple Sequence Repeat (SSR) |
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