| 猪链球菌病二联灭活疫苗的研制 |
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| 引用本文： | 王建,刘佩红,陆承平,王永康,张苏华,沈素芳,沈莉萍.猪链球菌病二联灭活疫苗的研制[J].南京农业大学学报,2003,26(1):70-73. |
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| 作者姓名： | 王建 刘佩红 陆承平 王永康 张苏华 沈素芳 沈莉萍 |
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| 作者单位： | 1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095
2. 上海市畜牧兽医站,上海,201103 |
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| 基金项目： | 国家 973项目子课题 (G19990 1190 6)，上海市科技兴农重点攻关项目 (农科攻字 98第 0 5 4号 ) |
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| 摘 要： | 以马莲球菌兽疫亚种（Stretococcus equi subsp.zooepidemicus)ATCC35246株和猪链球菌2型（S.suis type2)HA9801株作为生产菌株，制备氢氧化铝胶二联灭活疫苗，经过无菌及安全检验合格，肌肉接种仔猪。每头2mL。免疫15d后，对HA9801和ATCC35246的保护率分别为100%（26/26）和92.3%(24/26)。用二联灭活苗免疫后4周内仔猪体内抗体水平持续上升，从第5周开始逐渐下降；再次免疫后，抗体水平迅速回升，120d后仍维持较高水平，攻毒保护试验和抗体消长规律的测定显示，制备的猪链球菌病二联灭活苗具有良好的免疫保护作用。
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| 关 键 词： | 猪 链球菌病 二联灭活疫苗 抗体 |
| 文章编号： | 1000-2030(2003)01-0070-04 |
| 修稿时间： | 2002年8月25日 |
Preparation of an inactivated combined vaccine against swine streptococcosis |
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| WANG Jian ,LIU Pei hong ,LU Cheng ping ,WANG Yong kang ,ZHANG Su hua SHEN Su fang ,SHEN Li ping.Preparation of an inactivated combined vaccine against swine streptococcosis[J].Journal of Nanjing Agricultural University,2003,26(1):70-73. |
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| Authors: | WANG Jian LIU Pei hong LU Cheng ping WANG Yong kang ZHANG Su hua SHEN Su fang SHEN Li ping |
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| Institution: | WANG Jian 1,LIU Pei hong 2,LU Cheng ping 1*,WANG Yong kang 2,ZHANG Su hua 2 SHEN Su fang 2,SHEN Li ping 2 |
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| Abstract: | A killed whole cell culture was prepared with Streptococcus equi subsp zooepidemicus ATCC35246 and S.suis type 2 HA9801.Piglets were inoculated intramusularly with 2 mL per animal at 28 day old.The protective efficacy reached 100%(26/26) and 92 3%(24/26) respectively after challenged with HA9801 and ATCC35246.The antibody titers increased persistently within 4 weeks and began to decline gradually since the week 5 post immunization.The titers were rised rapidly after secondary immunization and perisisted at a higher lever at 120 day.The results showed that the inactivated combined vaccine could provide a protection against the infection of S.suis type 2 and S.equi subsp. zooepidemicus. |
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| Keywords: | swine inactivated combined vaccine streptococcosis antibody |
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