| PMSG和hCG对猪卵母细胞体外成熟的影响 |
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| 引用本文： | 蔡令波,王锋,顾熟琴,杭苏琴,牛树理.PMSG和hCG对猪卵母细胞体外成熟的影响[J].畜牧与兽医,2002,34(12):1-3. |
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| 作者姓名： | 蔡令波 王锋 顾熟琴 杭苏琴 牛树理 |
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| 作者单位： | 南京农业大学动物胚胎工程技术中心,江苏,南京,210095 |
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| 摘 要： | 对猪卵母细胞不同发育阶段的激素需要进行了初步探讨。结果表明 :猪卵母细胞体外培养 48h ,前 2 4h在培养液中加入激素 ,后 2 4h不加激素 ,卵母细胞的A级成熟率 (51 73% )和总成熟率 (83 2 5 % )最高 ,极显著高于前 2 4h不加激素 ,后 2 4h添加激素培养的成熟率 (P <0 0 1 ) ;也显著高于不含激素的培养液连续培养 48h的成熟率 (P <0 0 5) ;但与添加激素连续培养 48h组成熟率差异不显著 (P >0 0 5)。
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| 关 键 词： | 猪 卵母细胞 体外成熟 激素 发育阶段 |
| 文章编号： | 0529-5130(2002)12-0001-03 |
| 修稿时间： | 2002年7月22日 |
Effects of PMSG and hCG on IVM of oocytes in porcine |
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| CAI Ling bo,WANG Feng ,GU Shu qin,HANG Su qin,NIU Shu li.Effects of PMSG and hCG on IVM of oocytes in porcine[J].Animal Husbandry & Veterinary Medicine,2002,34(12):1-3. |
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| Authors: | CAI Ling bo WANG Feng GU Shu qin HANG Su qin NIU Shu li |
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| Institution: | CAI Ling bo,WANG Feng *,GU Shu qin,HANG Su qin,NIU Shu li |
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| Abstract: | The hormone requirement in different developmental stages of porcine oocytes was studied. The results showed that when oocyte maturation for 48h in vitro, removal of hormone supplements from mature media at 24h after culture enhance oocyte maturation and cumulus expansion. The maturation rate (83 25%) was higher than that of oocytes that were cultured for 24h in the media without hormone subsequent supplement hormone for another 24h (63 94%) (P<0 01), and also higher than that of oocytes culture for 48h in media without hormones (60 01%) (P<0 05). |
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| Keywords: | porcine oocyte hormone IVM development stage |
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