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| 贵州地方稻种资源的ISSR遗传多样性评价 | |
| 引用本文： | 范富华,施文娟,阮仁超,赵德刚,文晓鹏.贵州地方稻种资源的ISSR遗传多样性评价[J].种子,2010,29(8). |
| 作者姓名： | 范富华 施文娟 阮仁超 赵德刚 文晓鹏 |
| 作者单位： | 1. 贵州大学/贵州省农业生物工程重点实验室,贵阳,550025 2. 贵州省种子管理站,贵阳,550001 3. 贵州省农作物品种资源研究所,贵阳,550006 |
| 基金项目： | 国家科技支撑计划课题 |
| 摘 要： | 利用ISSR标记对27份贵州地方稻种资源进行了遗传多样性鉴定与评价。结果表明,用筛选出的12条引物进行PCR扩增,共扩增出清晰、重复性好的谱带155个,其中127个具有多态性,比率为81.9%。ISSR指纹图谱表明,3个引物就能有效地将27份稻种资源完全区别;聚类分析表明,供试品种间的遗传相似性系数为0.555～0.942。在相似性系数为0.66时可将供试种基本按糯性与非糯性分为两大类群。 |
| 关 键 词： | 贵州 地方稻种 ISSR标记 遗传多样性 |
Evaluation on Genetic Diversity of Local Rice Resources in Guizhou by ISSR Markers |
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| FAN Fu-hua,SHI Wen-juan,RUAN Ren-chao,ZHAO De-gang,WEN Xiao-peng.Evaluation on Genetic Diversity of Local Rice Resources in Guizhou by ISSR Markers[J].Seed,2010,29(8). | |
| Authors: | FAN Fu-hua SHI Wen-juan RUAN Ren-chao ZHAO De-gang WEN Xiao-peng |
| Institution: | FAN Fu-hua1,SHI Wen-juan2,RUAN Ren-chao3,ZHAO De-gang1,WEN Xiao-peng1(1.Guizhou Key Laboratory of Agricultural Bioengineering,Guiyang 550025,China,2.Guizhou Station of Seed Management,Guiyang 550001,3.Guizhou Institute of Crop Genetic Resources,Guiyang 550006,China) |
| Abstract: | The identification and genetic diversity evaluation of 27 rice cultivars originated from Guizhou were carried out by ISSR markers.Twelve primers were used to amplify PCR.A total of 155 bands were yielded,among which 127 were polymorphic,taking up 81.9%.Three primers were employed to amplify genome DNAs of 27 cultivars effectively based on the fingerprints.The genetic similarity coefficients among the tested culti-vars were from 0.555 to 0.942.The systematical cluster analysis showed that 27 cultivars of loc... |
| Keywords: | Guizhou traditional rice(Oryza Sativa L ) germplasm ISSR genetic diversity |
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