| 共转化法剔除转基因小麦中的bar基因 |
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| 引用本文： | 张新梅,徐惠君,杜丽璞,叶兴国,辛志勇,郭蔼光,薛崧,马有志.共转化法剔除转基因小麦中的bar基因[J].作物学报,2004,30(1):26-30. |
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| 作者姓名： | 张新梅 徐惠君 杜丽璞 叶兴国 辛志勇 郭蔼光 薛崧 马有志 |
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| 作者单位： | 中国农业科学院作物育种栽培研究所，农业部作物遗传育种重点实验室，北京100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用PCR检测了268株转小麦黄花叶病病毒复制酶基因T3代植株，初步筛选出只含有功能基因(WYMV-Nib8基因)而不含有筛选标记基因(bar基因)的转基因小麦植株28棵，为转基因小麦的安全性种植提供了保障。同时对无标记基因的转基因植株进行了叶片涂抹除草剂验证，探讨了叶片涂抹除草剂结合目的基因PCR检测筛选无选择标记转基因植株
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| 关 键 词： | 小麦 转基因 共转化 安全性 |
| 收稿时间： | 2003-03-06 |
| 修稿时间： | 2003年3月6日 |
Excision of bar Gene from Transgenic Wheat Obtained by Biolistic Co-transformation |
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| ZHANG Xin Mei ,XU Hui Jun ,DU Li Pu ,YE Xing Guo ,XIN Zhi Yong ,GUO Ai Guang ,XUE Song ,MA You Zhi.Excision of bar Gene from Transgenic Wheat Obtained by Biolistic Co-transformation[J].Acta Agronomica Sinica,2004,30(1):26-30. |
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| Authors: | ZHANG Xin Mei XU Hui Jun DU Li Pu YE Xing Guo XIN Zhi Yong GUO Ai Guang XUE Song MA You Zhi |
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| Institution: | Institute of Crop Breeding and Cultivation CAAS, Ministry Key Lab for Crop Genetics and Breeding, Beijing 100081 |
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| Abstract: | Transgenic crops have developed in commercialized scale in some countries, but their biosafety have attracted public concerns in the world at the same time. Therefore, it is becoming more and more important to eliminate the selective genes because of their negative functions, such as antibiotics resistance and herbicide resistance. 268 T 3 transgenic wheat plants of Yangmai158 containing wheat yellow mosaic virus resistance gene and bar selection gene, which were obtained by biolistic particle co transformation, were analyzed by PCR in this study, and 28 plants only with the target gene were identified finally. Moreover, leaf painting of herbicide was applied to confirm the marker free transgenic plants. It could be more efficient to combine leaf painting and PCR to select bar free transgenic wheat. |
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| Keywords: | Wheat Transgenic plant Co transformation Biosafety |
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