| 进口动物产品中莱克多巴胺残留的快速检测 |
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| 引用本文： | 陈茹.进口动物产品中莱克多巴胺残留的快速检测[J].中国兽药杂志,2011,45(8):20-23. |
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| 作者姓名： | 陈茹 |
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| 作者单位： | 广东出入境检验检疫局技术中心 |
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| 基金项目： | 致谢：感谢广东出入境检验检疫局技术中心林峰研究员和广州恩康科技有限公司提供技术支持. |
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| 摘 要： | 应用经验证可靠的ELISA方法，对进口猪产品进行莱克多巴胺残留快速筛选检测，对ELISA检测阳性的样品采用色谱质谱联用分析方法进行确证检测。1330份猪产品的检测结果显示，采用ELISA方法检出阳性率达11．4％（152／1330）；对ELISA阳性样品采用高效液相色谱-串联质谱（HPLC—MS／MS）或气相色谱-质谱方法（GC—MS）确证，阳性率达90．8％（138／152）。检测工作表明，综合运用快速筛选检验技术与确证检测技术可实现快速准确检测动物产品中莱克多巴胺残留，提高进出境检验工作效率。
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| 关 键 词： | 莱克多巴胺 猪产品 ELISA 高效液相色谱-串联质谱 气相色谱-质谱 |
| 收稿时间： | 6/9/2011 12:00:00 AM |
| 修稿时间： | 2011/6/27 0:00:00 |
Rapid detection of Ractopamine residues in imported animal products |
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| CHEN RU.Rapid detection of Ractopamine residues in imported animal products[J].Chinese Journal of Veterinary Drug,2011,45(8):20-23. |
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| Authors: | CHEN RU |
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| Institution: | Technical centre of Guangdong Entry-exit Inspection and Qurantine Bureau |
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| Abstract: | A validated ELISA method was applied to screening detection of ractopamine residues on imported porcine products, and positive samples were further determined by chromatography confirmatory analysis method. The ELISA detected 11.4 % (152/1330) positive from a total of 1330 porcine raw products, and 90.8% (138/152) samples were confirmed as positive by HPLC-MS/MS or GC-MS methods. The combined application of rapid screening method and confirmation method could achieve rapid and accurate detection of ractopamine residues on animal products and help improve the import and export inspection practices. |
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| Keywords: | Ractopamine animal products ELISA HPLC-MS/MS GC-MS/MS |
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