| 猪瘟病毒强弱毒株通用性RT-PCR检测方法研究进展 |
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| 引用本文： | 杨宗照,王剑峰,谢正,李洪洲,陈阳.猪瘟病毒强弱毒株通用性RT-PCR检测方法研究进展[J].动物检疫,2012(11):71-76. |
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| 作者姓名： | 杨宗照 王剑峰 谢正 李洪洲 陈阳 |
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| 作者单位： | [1]杭州市畜牧兽医局,浙江杭州310020 [2]杭州市原种场,浙江杭州311115 [3]杭州市萧山区畜牧兽医局,浙江杭州311203 |
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| 摘 要： | 猪瘟是一种严重危害养猪业的重大传染病，为了更好的防治本病，及时诊断尤为重要。猪瘟的诊断方法包括病毒分离、RT-PCR技术等。文章主要对猪瘟病毒基因结构、用于猪瘟检测的普通RT-PCR、荧光定量RT-PCR技术以及它们的优缺点和采样注意事项等作了概述。普通RT-PCR方法比较便宜但费力，而荧光定量RT-PCR方法恰恰相反，比较昂贵但省力。猪瘟检测的趋势是自动化和快速并最好同时能够区分野毒和疫苗株。
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| 关 键 词： | 猪瘟 猪瘟病毒 RT-PCR 荧光定量 聚合酶链反应 |
Advances in Research of General RT-PCR for Detection of Virulent and Weak Swine Fever Virus Strains |
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| Authors: | Yang Zongzhao Wang Jianfeng Xie Zheng Li Hongzhou Chen Yang |
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| Institution: | 1.Hangzhou Husbandry and Veterinary Bureau, Hangzhou, Zhejiang, 310020, China： 2.Hangzhou Swine Breeding Farm, Hangzhou, Zhejiang, 311115, China： 3.The Husbandry and Veterinary Bureau of Xiaoshan District, Hangzhou, Zhejiang, 311203, China) |
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| Abstract: | Classical swine fever （CSF） is an important pig disease, endangering the pig industry and causing large economic losses each year.In order to better control and prevention of this disease, timely diagnosis is very important.The diagnosis of CSFV includes virus isolation, RT-PCR and so on.This review focuses on the CSFV gene structure and its application in detection methods： conventional RT-PCR and real time RT-PCR.The conventional RT-PCR is inexpensive, but is laborious; the latter is just the reverse-- need expensive fluorescent PCR machine but is quick and easy to do.The tendency of CSF diagnosis is automation and rapidness and can differentiate the wild-type CSFV from vaccine strains such as HCLV. |
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| Keywords: | Classic swine fever RT-PCR examination method |
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