| 应用RAPD技术快速鉴定蔬菜上的弯孢类炭疽菌 |
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| 引用本文： | 曾大兴,戚佩坤,姜子德.应用RAPD技术快速鉴定蔬菜上的弯孢类炭疽菌[J].植物病理学报,2002,32(2):170-174. |
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| 作者姓名： | 曾大兴 戚佩坤 姜子德 |
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| 作者单位： | 华南农业大学资环学院真菌室, 广州 518055 |
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| 摘 要： | 应用筛选的14个随机引物,对分离自广州不同蔬菜上的12个弯孢炭疽菌菌株进行RAPD扩增。所有引物对分离自辣椒、香葱等蔬菜上的11个菌株都产生非常相似甚至一致的扩增带型,仅来自姜上的菌株的带型显著不同;UPGMA聚类分析将12个菌株聚为2群,其中11个菌株(Cap1~Cap11)以很高的Dice相似系数(>82%)组成一群,姜上的菌株(Cap12)为另一群。根据这些结果,将上述来自辣椒等蔬菜上的11个菌株鉴定为同一个种——辣椒炭疽菌(Colletotrichum capsici);而姜上的炭疽菌可能是另一个种。结果不支持将来自葱属植物的3个菌株鉴定为葱炭疽菌(C.circinans)。
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| 关 键 词： | 辣椒炭疽菌 RAPD分析 |
| 文章编号： | 0412-0914(2002)02-0170-05 |
| 修稿时间： | 2000年11月17 |
IDENTIFICATION OF THE FALCATE Colletotrichum ON VEGETABLE USING RAPD ANALYSIS |
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| ZENG Da xing,QI Pei kun,JIANG Zi de.IDENTIFICATION OF THE FALCATE Colletotrichum ON VEGETABLE USING RAPD ANALYSIS[J].Acta Phytopathologica Sinica,2002,32(2):170-174. |
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| Authors: | ZENG Da xing QI Pei kun JIANG Zi de |
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| Institution: | College of Natural Resources & Environment, South China Agric. Univ., Guangzhou 510642, China |
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| Abstract: | The DNA of 12 falcate Colletotrichum strains from different vegetable in Guangzhou was amplified with 14 selected Operon primers. RAPD patterns generated from all primers were high similar or identical for 11 of the 12 strains, only pattern of 1 isolate from Zingiber officinale was different clearly. UPGMA analysis showed that 12 strains were divided into two groups. Eleven of the 12 strains clustered into groupⅠ, and the strain from Z.officinale formed groupⅡ. Base on these results, the 11 strains were identified as a distinct species-C. capsici, and the strain on Z. officinale may represent another species. These results didn't support that 3 strains originated from the genus Allius hosts belonged to C.circinans. |
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| Keywords: | Colletotrichum capsici RAPD analysis |
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