| 农杆菌介导高粱遗传转化的相关因素优化 |
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| 引用本文： | 张微,王良群,刘勇,郝艳芳,杨伟,白鸿雁,武擘.农杆菌介导高粱遗传转化的相关因素优化[J].作物杂志,2018,34(1):56-248. |
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| 作者姓名： | 张微 王良群 刘勇 郝艳芳 杨伟 白鸿雁 武擘 |
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| 作者单位： | 1 高粱遗传与种质创新山西省重点实验室,030600,山西晋中2 山西省农业科学院高粱研究所,030600,山西晋中 |
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| 基金项目： | 山西省科技攻关项目(20150311003-2);高粱遗传与种质创新山西省重点实验室青年基金(2015Q-06) |
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| 摘 要： | 为建立一个稳定的高粱遗传转化体系,以高粱RHMC品种为试验材料,采用内含有P CAMBIA3301质粒载体的根癌农杆菌EHA105介导的方法研究了高粱遗传转化的相关因素。结果表明,外植体预培养2d,GUS表达效果最好;在侵染液和共培养基中加入200μmol/L乙酰丁香酮时,GUS表达效果最好;侵染液浓度OD600值的适宜范围为0.8~1.0;农杆菌侵染愈伤组织的适宜时间为5~10min;共培养的适宜时间为2d。初步建立了一套高粱遗传转化体系,为今后高粱转化提供一些参考。
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| 关 键 词： | 高粱 农杆菌 遗传转化 愈伤组织 |
| 收稿时间： | 2017-11-01 |
Optimization of the Factors Related to the Efficiency of Agrobacterium-Mediated Transformation of Sorghum |
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| Wei Zhang,Liangqun Wang,Yong Liu,Yanfang Hao,Wei Yang,Hongyan Bai,Bo Wu.Optimization of the Factors Related to the Efficiency of Agrobacterium-Mediated Transformation of Sorghum[J].Crops,2018,34(1):56-248. |
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| Authors: | Wei Zhang Liangqun Wang Yong Liu Yanfang Hao Wei Yang Hongyan Bai Bo Wu |
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| Institution: | 1 Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Jinzhong 030600, Shanxi, China2 Sorghum Institute, Shanxi Academy of Agricultural Sciences, Jinzhong 030600, Shanxi, China |
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| Abstract: | Using sorghum variety RHMC and the agrobacterium strain EHA105 containing P CAMBIA3301 plasmid as the materials, we studied the factors which influence agrobacterium-mediated transformation efficiency of the sorghum so as to establish a stable transformation system. The results showed that when the explants were pre-cultured for 2d, the GUS staining was the best. When 200μmol/L acetosyringone was added to infecting fluid and co-culture media, the GUS staining was the best. The suitable concentration range of the OD600 was 0.8-1.0. The suitable time of the infection with agrobacterium was 5-10min. The suitable time of the co-culture was 2d. In this study, a stable transformation system was preliminary established. This provided some references for the transformation of sorghum in the future. |
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| Keywords: | Sorghum Agrobacterium Genetic transformation Callus |
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