| 碱预处理玉米芯生物转化L-乳酸 |
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| 引用本文： | 张丽,陈晓佩,李鑫,余世袁,勇强.碱预处理玉米芯生物转化L-乳酸[J].林产化学与工业,2016(2):51-56. |
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| 作者姓名： | 张丽 陈晓佩 李鑫 余世袁 勇强 |
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| 作者单位： | 南京林业大学林业资源高效加工利用协同创新中心，江苏 南京 210037; 南京林业大学化学工程学院，江苏 南京 210037 |
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| 基金项目： | “十二五”国家科技支撑计划资助(2015BAD15B09)，江苏高校自然科学研究重大项目(14KJA220003)，江苏省普通高校研究生科研创新计划项目(CXZZ13_0544) |
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| 摘 要： | 玉米芯经碱预处理后,采用米根霉对其发酵制备L-乳酸,同时考察分步糖化发酵(SHF)和同步糖化发酵(SSF)两种工艺。实验结果表明,水洗碱预处理玉米芯酶水解性能优于未水洗碱预处理玉米芯,水洗过程可显著提高米根霉发酵性能。分步糖化发酵工艺下,米根霉于40℃下发酵48 h,可将含有31.84 g/L葡萄糖、6.38 g/L木糖的酶解液转化为14.65 g/L的L-乳酸,L-乳酸得率为0.29 g/g(以绝干物料计,下同);同步糖化发酵工艺下,米根霉40℃发酵36 h将底物质量浓度为50 g/L的水洗碱预处理玉米芯高效转化为L-乳酸,L-乳酸得率为0.44 g/g。
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| 关 键 词： | 米根霉 碱预处理玉米芯 L-乳酸 分步糖化发酵 同步糖化发酵 |
Production of L-Lactic Acid by Bioconversion of Alkali Pretreated Corncob |
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| Abstract: | Corncob pretreated by alkali was used as renewable raw material for L-lactic acid production by Rhizopus oryzae. The processes of separate saccharification and fermentation ( SHF) and simultaneous saccharification and fermentation ( SSF) were studied. The results showed that the enzymatic hydrolysis performance of washed alkali pretreated corncob was better than that of unwashed alkali pretreated corncob. Water-washing process could enhance the fermentability of alkali pretreated corncob. The washed alkali pretreated corncob hydrolysate with 31. 84 g/L glucose and 6. 38 g/L xylose was converted to 14. 65 g/L of L-lactic acid which was equivalent to a yield of 0. 29 g/g dry biomass with SHF process at 40 ℃ for 48 h. In contrast, a higher L-lactic acid yield (0. 44 g/g dry biomass) was achieved at 40 ℃ for 36 h, when the substrate concentration of 50 g/L washed alkali pretreated corncob was used in SFF process. |
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| Keywords: | Rhizopus oryzae alkali pretreated corncob L-lactic acid separate saccharification and fermentation simultaneous saccharification and fermentation |
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