| 火炬松松针多糖提取工艺及其抗氧化性研究 |
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| 引用本文： | 许小向,尹小莉,洪艳平,邹凯,上官新晨,梁强.火炬松松针多糖提取工艺及其抗氧化性研究[J].林产化学与工业,2016(3):114-120. |
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| 作者姓名： | 许小向 尹小莉 洪艳平 邹凯 上官新晨 梁强 |
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| 作者单位： | 1. 江西农业大学食品科学与工程学院，江西省天然产物与功能食品重点实验室，江西 南昌 330045;2. 江西农业大学图书馆,江西 南昌,330045;3. 江西农业大学食品科学与工程学院，江西省天然产物与功能食品重点实验室，江西 南昌 330045; 江西省食品药品监督管理局，江西 南昌 330045 |
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| 基金项目： | 国家科技支撑计划项目(2013BAD10B04-3)；江西省高等学校科技落地计划项目(KJLD12021) |
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| 摘 要： | 以多糖得率为指标,从火炬松松针中浸提多糖,并测定其体外抗氧化活性。在单因素试验基础上采用响应面法优化超声波辅助热水浸提火炬松松针多糖,最佳的工艺参数为:30 g松针粉末在超声波作用时间25 min,热水浸提1 h,液料比25∶1(m L∶g),热水浸提温度91℃。在此条件下,火炬松松针多糖得率达1.867%,提取率达91.39%。通过测定松针多糖对苯基苦基肼自由基(DPPH·)、羟基自由基(·OH)和2,2-联氮-二-(3-乙基-苯并噻唑-6-磺酸)自由基(ABTS+)的清除能力评价其体外抗氧化能力。结果显示:火炬松松针多糖对自由基DPPH·、·OH和ABTS+都有较强清除能力,且都呈较好的量效关系,火炬松松针多糖具有较强的体外抗氧化能力。
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| 关 键 词： | 火炬松 松针多糖 超声波辅助提取 响应面法 抗氧化性 |
Extraction Process and Antioxidant Activity of Pinus taeda L.Polysaccharides |
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| Abstract: | With the polysaccharides yield as target , the polysaccharides from pine needles of Pinus taeda L.was extracted and its antioxidant activity in vitro was determined .The ultrasonic-assisted extraction technology of polysaccharides from pine needles of P.taeda was confirmed by using Box-Behnken response surface methodology .The results showed that the optimal extraction process conditions were ultrasonic time 25 min, water extraction time 1 h, liquid-material ratio of 25∶1 ( mL∶g) and extraction temperature 91 ℃.Under optimal conditions , the yield of polysaccharides was up to 1.867%.The antioxidant activity in vitro was evaluated by measuring the DPPH· , · OH and ABTS + free radical removal ability.As a result, the pine needle polysaccharides had a relatively strong capacity to scavenge DPPH ,OH and ABTS +free radical .They had a better concentration-response relationship .It showed that pine needle polysaccharides had an obvious antioxidant activity . |
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| Keywords: | Pinus taeda L pine needle polysaccharides ultrasonic-assisted extraction response surface methodology antioxidant activity |
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