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尾叶紫薇开花及花粉萌发研究
引用本文:王晓玉,徐婉,胡杏,蔡明,潘会堂,张启翔.尾叶紫薇开花及花粉萌发研究[J].浙江林学院学报,2012,29(6):966-970.
作者姓名:王晓玉  徐婉  胡杏  蔡明  潘会堂  张启翔
作者单位:1. 北京林业大学园林学院,北京,100083
2. 北京林业大学园林学院,北京100083;国家花卉工程技术研究中心,北京100083
基金项目:"十二五"国家科技支撑计划项目,北京市园林绿化局计划项目,北京市共建项目专项资助
摘    要:通过观测尾叶紫薇Lagerstroemiacaudate花朵的开放过程,并采用离体萌发法测定花粉生活力,研究了尾叶紫薇的开花习性、花粉萌发及储藏条件。结果表明:凌晨3:30尾叶紫薇花萼开裂;6:00花瓣全部展平;6:30花药开始开裂。柱头上开始分泌黏液,进行杂交育种时须于6:30前去雄;6:30—7:30是授粉的最佳时间。采用正交试验方法比较蔗糖、聚乙二醇4000(PEG 4000)、硼酸和氯化钙对尾叶紫薇花粉的离体萌发率的影响。结果表明:最佳离体培养基为100mg·L-1蔗糖+30mg·L-1硼酸+30mg·L-1氯化钙+150g·L-1PEG 4000,花粉萌发率可达96.28%。尾叶紫薇花粉在自然条件下储藏2d后生活力急剧下降,6d后花粉完全失活,4℃是花粉储藏的最佳温度,保存9d仍能保持30%的萌发率.在散粉后30d失活。图2表2参15

关 键 词:植物学  尾叶紫薇  开花习性  花粉萌发  花粉储藏

Florescence and pollen germination in Lagerstroemia caudate
WANG Xiao-yu , XU Wan , HU Xing , CAI Ming , PAN Hui-tang , ZHANG Qi-xiang.Florescence and pollen germination in Lagerstroemia caudate[J].Journal of Zhejiang Forestry College,2012,29(6):966-970.
Authors:WANG Xiao-yu  XU Wan  HU Xing  CAI Ming  PAN Hui-tang  ZHANG Qi-xiang
Institution:1. College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China; 2. China National Engineering Research Center for Floriculture, Beijing 100083, China)
Abstract:In order to study the flowering habit and the conditions for pollen germination and storage of Lager stroemia caudate, the processes of flower opening and pollen viability of Lagerstroemia caudate were observed over a 30 d period. Pollen germination was also tested using orthogonal test of PEG 4000, boric acid, CaCI2, and sucrose. Results showed that at 3:30 sepals of L. caudate started to diverge, at 6:00 petals flattened up, at 6:30 the anther diverged, and at 06:30 the stigma secreted a large amount of mucus. The highest rate of in vitro pollen germination which could reach 96.28% was with sucrose (100 g·L-1) + boric acid (30 mg·L-1) + CaCI2 (30 rag· L-1) + PEG 4000 (150 g· L-1). Pollen germination in the indoor natural environment deereased sharply from 87% to 65% in 2 d with the pollen losing vigor in 6 d. The best preservation temperature for dry pollen was 4 ℃, in which temperature the dry pollen viability could keep 30%, with dry pollen losing its vig- or in 30 d. Thus, as a basis for breeding, if L. caudate was used as a female parent in hybridization, the sta- mens must be removed before 6:30, and the best pollination time would be at 7:30. Ch, 2 fig. 2 tab. 15 ref. ]
Keywords:botany  Lagerstroemia caudate  flowering habit  pollen germination  pollen storage
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