| 鸡传染性支气管炎X毒株S1基因的克隆与真核表达 |
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| 引用本文： | 柏佳宁,马同锁,赵宝华.鸡传染性支气管炎X毒株S1基因的克隆与真核表达[J].中国兽医学报,2006,26(2):122-125. |
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| 作者姓名： | 柏佳宁 马同锁 赵宝华 |
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| 作者单位： | 1. 河北师范大学,生命科学学院,河北,石家庄,050016
2. 河北经贸大学,生物科学与工程学院,河北,石家庄,050061 |
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| 基金项目： | 河北省教育厅博士科研项目 |
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| 摘 要： | 根据GenBank中报道的鸡传染性支气管炎病毒（IBV）S1基因序列，设计了1对引物，克隆了IBV强毒株X的S1基因，基因大小为1．62kb。将其导入真核表达载体系统pIRES1neo中，表达蛋白为61000。
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| 关 键 词： | 鸡传染性支气管炎病毒 S1基因 基因克隆 基因表达 |
| 文章编号： | 1005-4545(2006)02-0122-04 |
| 收稿时间： | 2005-02-16 |
| 修稿时间： | 2005年2月16日 |
Cloning and Eukaryotic Expression of the S1 Gene of IBV Nephropathogenic Strain X |
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| BAI Jia-ning,MA Tong-suo,ZHAO Bao-hua.Cloning and Eukaryotic Expression of the S1 Gene of IBV Nephropathogenic Strain X[J].Chinese Journal of Veterinary Science,2006,26(2):122-125. |
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| Authors: | BAI Jia-ning MA Tong-suo ZHAO Bao-hua |
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| Institution: | 1. College of Life Seience,Hebei Normal University, Shijiazhuang 050016, China; 2. College of Biology Science and Biotechnology, Hebei Economic and Trade University, Shijiazhuang 050061, China |
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| Abstract: | A pair of primers for amplifying the S1 gene of avian infectious bronchitis virus(IBV) was designed,with which the predicted 1.62 kb fragment had been amplified by RT-PCR from Strain X.Then the expression vectors containing the S1 gene of X strain was constructed and the recombinate expression protein obtained.The expressed S1 protein was identified by ELISA,Western blotting and indirect fluorescent antibody technique and the biological characters of S1 protein was identified. |
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| Keywords: | IBV S1 gene gene cloning gene expression |
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