| 补骨脂水提液对成骨细胞OPG-RANKL mRNA表达的影响 |
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| 引用本文： | 田丹,刘钟杰,赵晓燕,李彩虹,王炼,邢晓旭,韩博.补骨脂水提液对成骨细胞OPG-RANKL mRNA表达的影响[J].中国兽医杂志,2011,47(12). |
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| 作者姓名： | 田丹 刘钟杰 赵晓燕 李彩虹 王炼 邢晓旭 韩博 |
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| 作者单位： | 中国农业大学动物医学院,北京 海淀,100193 |
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| 摘 要： | 为了探讨补骨脂水提液对体外培养大鼠成骨细胞核因子-KB受体活化因子配体(RANKL)和骨保护素(OPG) mR-NA表达的影响,选取出生24 h内的SD大鼠颅盖骨,依次用胰酶和胶原酶消化分离培养成骨细胞,用不同浓度的补骨脂水提液(10-1,1.0 mg/mL和10 mg/mL)作用第三代细胞,分别在药物作用24、48 h和72 h后提取细胞总RNA,用实时荧光定量PCR法检测细胞中RANKL和OPG mRNA.结果显示,补骨脂水提液各浓度均能不同程度地促进成骨细胞OPG mRNA的表达、抑制RANKLmRNA的表达,从而上调OPG/RANKL mRNA比值,其中1.0 mg/mL和10 mg/mL的补骨脂水提液作用显著.说明补骨脂水提液通过调节成骨细胞分泌OPG和RANKL的量来促进骨形成、抑制骨吸收,从而治疗骨质疏松.
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| 关 键 词： | 补骨脂 成骨细胞 核因子-kB受体活化因子配体 骨保护素 |
Effect of Malaytea Scurfpea Fruit extracts on the expression of OPG and RANKL mRNA in rat osteoblastsin vitro |
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| TIAN Dan
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LIU Zhong-jie
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ZHAO Xiao-yan
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LI Cai-hong
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WANG Lian
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XING Xiao-xu
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HAN Bo.Effect of Malaytea Scurfpea Fruit extracts on the expression of OPG and RANKL mRNA in rat osteoblastsin vitro[J].Chinese Journal of Veterinary Medicine,2011,47(12). |
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| Authors: | TIAN Dan LIU Zhong-jie ZHAO Xiao-yan LI Cai-hong WANG Lian XING Xiao-xu HAN Bo |
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| Institution: | TIAN Dan,LIU Zhong-jie,ZHAO Xiao-yan,LI Cai-hong,WANG Lian,XING Xiao-xu,HAN Bo (College of Veterinary Medicine,China Agricultural University,Beijing 100193,China) |
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| Abstract: | The objective of this study was to investigate the effect of Malaytea Scurfpea Fruit extracts on the expression of RANKL and OPG mRNA in rat osteoblasts.The osteoblasts were derived from calvariae of neonatal rat and digested by trypsin and collagenase.The cultured osteoblasts of the third-generation were exposed to different concentrations of Malaytea Scurfpea Fruit extracts(10-1,1.0 and 10mg/mL).Total RNA was prepared from osteoblasts after 24,48 and 72 h,respectively,and the expression of RANKL and OPG m... |
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| Keywords: | Malaytea Scurfpea Fruit osteoblast RANKL OPG RT-PCR |
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