| 通过农杆菌介导把Harpin_(Ea)基因导入马铃薯的初步研究 |
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| 引用本文： | 李先平,何云昆,赵志坚,李树莲.通过农杆菌介导把Harpin_(Ea)基因导入马铃薯的初步研究[J].中国马铃薯,2002,16(4):199-202. |
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| 作者姓名： | 李先平 何云昆 赵志坚 李树莲 |
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| 作者单位： | 1. 云南省农科院生物技术研究所,昆明,650223
2. 云南省农科院植保所 |
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| 基金项目： | 云南省九五科技攻关资助项目———马铃薯生物技术育种研究 |
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| 摘 要： | 应用农杆菌介导的叶盘法进行马铃薯遗传转化研究。通过D3、D4培养基筛选法 ,把HarpinEa蛋白基因导入马铃薯四倍体栽培种大西洋 (Atlantic) ,获得 10 7个转化株系。并经过卡那霉素的抗性鉴定和PCR分子检测 ,有 5 9株PCR检测呈阳性 ,占所有转化植株的 5 5 14%。结果表明HarpinEa基因已整合到马铃薯的基因组中。转化植株的抗病性鉴定正在进行中。
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| 关 键 词： | HarpinEa基因 马铃薯晚疫病 转基因植株 |
| 文章编号： | 1001-0092(2002)04-199-04 |
| 修稿时间： | 2002年3月25日 |
ANALYSIS OF LATE-BLIGHT RESISTANCE IN TRANSGENIC POTATO EXPRESSING HARPINEa GENE |
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| LI Xian-ping ,HE Yun-kun ,ZHAO Zhi-jian ,LI Shu-lian.ANALYSIS OF LATE-BLIGHT RESISTANCE IN TRANSGENIC POTATO EXPRESSING HARPINEa GENE[J].Chinese Potato,2002,16(4):199-202. |
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| Authors: | LI Xian-ping HE Yun-kun ZHAO Zhi-jian LI Shu-lian |
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| Institution: | LI Xian-ping 1,HE Yun-kun 1,ZHAO Zhi-jian 1,LI Shu-lian 2 |
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| Abstract: | Potato commercial cultivar Atlantic was transformed with Harpin Ea gene via Agrobacterium tumefaciens mediate gene transfer using leaf disk method.107 regenerated potato plants were obtained through D3 and D4 selection medium.Kanamycin resistance and PCR amplification analysis showed that 59 putative transgenic plants had the Harpin Ea gene,and the transformation ratio was 55.14 percent.The Late-blight resistance of potato putative transgenic plants in laboratory and in field will be tested in the year 2002. |
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| Keywords: | Harpin Ea gene potato late blight transgenic plants |
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