| In vitro antioxidant activity of callus culture of carrot （Daucus carom） |
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| 作者姓名： | Tadhani Manish ;Patel vinayak ;Rema Subhash |
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| 作者单位： | [1]Rayat London College, Middlesex TW5 OLJ, UK; [2]Food Biotechnology Laboratory,Post Graduate Department of Home Science,Sardar Patel University,Vallabh Vidyanagar-388 120 Gujarat,India |
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| 摘 要： | Carrot （Daucus carom） is a valuable source of health promoting ingredients such as anthocyanin, carotenes, phenolic compounds etc. These substances are important to man as a source of pharmaceuticals, fragrance, agrochemicals as well as food additives and used for prevention of many chronic diseases. Since these activities may be correlated with the presence of antioxidant compounds, extract of carrot and carrot callus were evaluated for their anthocyanin, flavonoids and total phenolic content as well as total antioxidant activity. Anthocyanin content was measured by spectrophotometric method. Total phenols and flavonoids were analyzed according to the Folin-Ciocalteu method and total antioxidant activity was assessed by ferric reducing/antioxidant power （FRAP） assay. Anthocyanin, flavonoids and total phenolic content were estimated to be 9.36 mg%, 46.96 mg% and 57.01 mg% for callus and 6.82 mg%, 32.96 mg% and 42.69 mg% for carrot, respectively, on fresh weight basis. The total antioxidant activity for the callus and carrot was found to be 51.13 mg, 118.77 mg, 91.08 mg and 140.08 mg equivalent and 79.40 mg, 184.44 mg, 141.43 mg and 217.52 mg equivalent to gallic acid, vitamin C, butylated hydroxyanisole （BHA） and trolox, respectively, when expressed per 100 g on fresh weight basis. The antioxidant activity of fresh carrot was found to be higher compared to its callus.
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| 关 键 词： | 愈伤组织培养 总抗氧化活性 胡萝卜素 丁基羟基茴香醚 抗氧化成分 体外 总酚含量 黄酮类化合物 |
In vitro antioxidant activity of callus culture of carrot （Daucus carom） |
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| Tadhani Manish,;Patel vinayak,;Rema Subhash.In vitro antioxidant activity of callus culture of carrot （Daucus carom）[J].Agricultural Science ＆ Technology,2009(12):19-24. |
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| Abstract: | carrot callus anthocyanin flavonoids phenoliccompounds antioxidant activity |
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| Keywords: | carrot callus anthocyanin flavonoids phenoliccompounds antioxidant activity |
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