| 基于白木香ESTs的沉香属SSR分子标记引物筛选 |
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| 引用本文： | 杨 云,张 争,陈 波等.基于白木香ESTs的沉香属SSR分子标记引物筛选[J].江西农业学报,2014(5):76-79. |
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| 作者姓名： | 杨 云 张 争 陈 波等 |
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| 作者单位： | [1]中国医学科学院、北京协和医学院药用植物研究所海南分所／海南省南药资源保护与开发重点实验室,海南万宁571533 [2]中国医学科学院、北京协和医学院药用植物研究所,北京100193 |
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| 基金项目： | 基金项目：国家科技支撑计划（2011BAI01B07）. |
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| 摘 要： | 通过对白木香样品的454GS FLX Titanium高通量测序,获取了大量白木香EST序列,从中搜索出956个SSR,根据引物设计标准,设计了44对EST-SSR引物。在合适的PCR反应体系下,以海南白木香样品的DNA为模板,对设计的EST-SSR引物进行筛选,发现有22对EST-SSR引物能较好地扩增出产物。在沉香属3个种9份样品中进一步对这些可扩增的引物进行多态性初步验证,所筛选引物均可在所有沉香属样品中使用,为后续白木香居群及沉香属植物遗传多样性分析奠定基础。
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| 关 键 词： | 白木香 EST-SSR标记 多态性引物 筛选 |
Screening of SSR Molecular Marker Primer in Aquilaria According to ESTs of Aquilaria sinensis |
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| YANG Yun,ZHANG Zheng,CHEN Bo,MENG Hui.Screening of SSR Molecular Marker Primer in Aquilaria According to ESTs of Aquilaria sinensis[J].Acta Agriculturae Jiangxi,2014(5):76-79. |
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| Authors: | YANG Yun ZHANG Zheng CHEN Bo MENG Hui |
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| Institution: | 1 ( 1. Hainan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences ＆ Peking Union Medical College; Hainan Provincial Key Laboratory of Conservation and Development of Southern Medicine Resources, Wanning 571533, China; 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Bei- jing 100193, China) |
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| Abstract: | A large amount of Aquilaria sinensis （Lour.） Gilg EST sequences were obtained through high -throughput sequen- cing by 45gGS FLX Titanium. From these EST sequences, 956 SSRs were selected. Forty - four pairs of EST - SSR primers were designed to amplify flanking SSRs from 956 selected SSR - ESTs. Twenty - two pairs of primers were confirmed to be efficient in A. sinensis. The preliminary validation of the polymorphism of these 22 pairs of primers was further carried out in 9 samples of 3 species in Aquilaria, and the resuhs showed that all these primers could be used in Aquilaria. This work lays a foundation for the consequent family analysis of A. sinensis and genetic diversity analysis of plants in Aquilaria. |
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| Keywords: | Aquilaria sinensiz EST- SSR marker Polymorphic primers Screening |
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