| 酶联免疫法测定水产品中呋喃唑酮代谢物残留量 |
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| 引用本文： | 葛怀礼,蒙君丽,宋荪阳,李爱军,董李学.酶联免疫法测定水产品中呋喃唑酮代谢物残留量[J].河北渔业,2014(8):25-27. |
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| 作者姓名： | 葛怀礼 蒙君丽 宋荪阳 李爱军 董李学 |
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| 作者单位： | 唐山市畜牧水产品质量监测中心,河北唐山063000 |
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| 基金项目： | 基金项目：水产养殖产业违禁药物检测技术集成与示范（唐山市重点科技支撑项目）（12120212A） |
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| 摘 要： | 为测定水产品中呋喃唑酮代谢物(AOZ)的残留量,采用2-硝基苯甲醛进行衍生,乙酸乙酯提取AOZ。通过样品中残留的AOZ和酶标板上预包被的抗原竞争抗体,加入酶标二抗后与底物显色,测得样品中AOZ的残留量。测定了四组水产品中AOZ的残留量,测定浓度均小于1μg/kg,结果为阴性。向空白样品中分别添加0.5μg/kg、1.5μg/kg和2μg/kg的AOZ标准品,回收率为90%、109%和101%。因此得出结论:采用酶联免疫法测定水产品中AOZ残留量操作简单,检测时间短,结果准确,是一种适合大批量水产样品筛选的检测方法。
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| 关 键 词： | 酶联免疫法 水产品 呋喃唑酮代谢物 残留量 |
Residues of Furazolidone Metabolites in Aquatic Products by ELISA |
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| GE Huai li,MENG Jun li,SONG Sun yang,LI Ai jun,DONG Li xue.Residues of Furazolidone Metabolites in Aquatic Products by ELISA[J].Hebei Fisheries,2014(8):25-27. |
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| Authors: | GE Huai li MENG Jun li SONG Sun yang LI Ai jun DONG Li xue |
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| Institution: | (Tangshan Livestock and Aquatic Products Quality Monitoring Center, Tangshan,063000) |
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| Abstract: | Objective ：ELISA for the analysis of furazolidone （AOZ） residues in aquatic products were determined .Method：Samples were derivatived with 2-nitrobenzaldehyde and extracted with ethylacetate .The AOZ and microtiter board antigenic competition antibody ,chromogenic substrate with labeled sencondary antibody ,determine the residues of AOZ samples .Results ：When the samples were spiked with 0 .5 μg/kg ,1 .5 μg/kg and 2 μg/kg of AOZ ,the recoveries were 90% ,109% and 101%respectively by ELISA .Conclusion ：ESLISA is a rapid and exact method for the screen of AOZ abundant samples in aquatic products . |
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| Keywords: | ELISA Aquatic product Furazolidone Residues |
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