| 苏云金芽胞杆菌菌株94的培养基优化及对4种鳞翅目幼虫的毒力 |
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| 引用本文： | 李芳,陈家骅,黄慧婷.苏云金芽胞杆菌菌株94的培养基优化及对4种鳞翅目幼虫的毒力[J].福建农林大学学报(自然科学版),1997(1). |
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| 作者姓名： | 李芳 陈家骅 黄慧婷 |
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| 作者单位： | 福建农业大学植物保护系，厦门市总工会 |
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| 基金项目： | 福建省自然科学基金资助项目 |
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| 摘 要： | 采用正交设计，获得了苏云金芽胞杆菌菌株９４（Ｂｔ９４）的最适液体培养基配方：葡萄糖质量浓度为０．７６ｋｇＬ－１、胰蛋白胨＋酵母浸膏为０．８ｋｇＬ－１、无机盐为０．１ｋｇＬ－１．采用该培养基进行摇床培养，活芽孢数可达１０１×１０１１个Ｌ－１．用摇瓶培养液对斜纹夜蛾和小菜蛾进行毒力测定，其ＬＣ５０分别为３５５．９９５１±２．６０９８和５８．２９０８±１．４２６２ｍＬＬ
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| 关 键 词： | 苏云金芽胞杆菌菌株94 培养基 毒力测定 |
Media optimization of Bacillus thuringiensis 94 and its virulence to four species of lepidoteran larvae |
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| Li Fang,Chen Jiahua,Huang Huiting.Media optimization of Bacillus thuringiensis 94 and its virulence to four species of lepidoteran larvae[J].Journal of Fujian Agricultural and Forestry University,1997(1). |
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| Authors: | Li Fang Chen Jiahua Huang Huiting |
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| Institution: | Li Fang 1 Chen Jiahua 1 Huang Huiting 2 |
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| Abstract: | Utilizing orthogonal design to screen the suitable media for Bacillus thuringiensis 94 (Bt94) was conducted. The result showed that the best media mass concentrations were glucose 0.KG-4]76 kg·L -1 , peptone+yeast extract 0.8 kg·L -1 , salt 0.1 kg·L -1 , 101×10 11 ·L -1 of spores could be produced by using the media. The virulence tests for Prodenia citura Fabricius and Plutella xylostella linne indicated that the LC 50 of Bt94 was 355.995 1 ±2.KG-4]609 8 and 58.290 8±1.426 2 mL·L -1 respectively. |
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| Keywords: | Bacillus thuringiensis 94 media virulence test |
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