| 抗噬菌体色氨酸酶基因工程菌的筛选 |
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| 引用本文： | 梅运军,牟艳华,石德太,胡纯,王文清.抗噬菌体色氨酸酶基因工程菌的筛选[J].湖北农业科学,2012,51(12):2596-2598. |
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| 作者姓名： | 梅运军 牟艳华 石德太 胡纯 王文清 |
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| 作者单位： | 1. 武汉工业学院化学与环境工程学院,武汉,430023
2. 湖北省恩施州科技局,湖北恩施,445000 |
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| 摘 要： | 利用自发突变、紫外诱变、协同进化、紫外耦合协同进化4种方法对色氨酸酶基因工程菌WD0801进行了抗噬菌体筛选.结果表明紫外耦合协同进化法具有较好的筛选效果,筛选的42株稳定遗传的抗性菌株中有23株的长势高于出发株,11株的酶活优于出发株.
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| 关 键 词： | 色氨酸酶基因工程菌 抗噬菌体 筛选 |
Screening of Phage-Resistant Tryptophanase Genetic Engineering Strains |
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| MEI Yun-jun
,
MU Yan-hua
,
SHI De-tai
,
HU Chun
,
WANG Wen-qing.Screening of Phage-Resistant Tryptophanase Genetic Engineering Strains[J].Hubei Agricultural Sciences,2012,51(12):2596-2598. |
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| Authors: | MEI Yun-jun MU Yan-hua SHI De-tai HU Chun WANG Wen-qing |
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| Institution: | 1(1.School of Chemical and Environmental Engineering,Wuhan Polytechnic University,Wuhan 430023,China; 2.Bureau of Science and Technology,Enshi Automonous Prefecture,Enshi 445000,Hubei,China) |
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| Abstract: | 4 methods,spontaneous mutation,UV mutagenesis,co-evolution,UV coupled co-evolution,were employed to screen Escherichia coli tryptophanase genetic engineering strains.The results showed that the screening efficiency of UV-coupled co-evolution method was the best.Among the 42 stable phage-resistant strains screened out,the growth vigor of 23 strains,and enzyme activity of 11 strains were higher than the original strain. |
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| Keywords: | tryptophanase genetic engineering strain phage-resistant screening |
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